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Yorodumi- PDB-2gmo: NMR-structure of an independently folded C-terminal domain of inf... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2gmo | ||||||
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Title | NMR-structure of an independently folded C-terminal domain of influenza polymerase subunit PB2 | ||||||
Components | Polymerase basic protein 2 | ||||||
Keywords | VIRAL PROTEIN / compact beta-structure | ||||||
Function / homology | Function and homology information symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity / host cell mitochondrion / 7-methylguanosine mRNA capping / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity / cap snatching / virion component / viral RNA genome replication / virus-mediated perturbation of host defense response / RNA-dependent RNA polymerase activity / DNA-templated transcription ...symbiont-mediated suppression of host mRNA transcription via inhibition of RNA polymerase II activity / host cell mitochondrion / 7-methylguanosine mRNA capping / symbiont-mediated suppression of host cytoplasmic pattern recognition receptor signaling pathway via inhibition of MAVS activity / cap snatching / virion component / viral RNA genome replication / virus-mediated perturbation of host defense response / RNA-dependent RNA polymerase activity / DNA-templated transcription / host cell nucleus / RNA binding Similarity search - Function | ||||||
Biological species | Influenza A virus | ||||||
Method | SOLUTION NMR / simulated annealing | ||||||
Authors | Boudet, J. / Tarendeau, F. / Guilligay, D. / Mas, P. / Bougault, C.M. / Cusack, S. / Simorre, J.-P. / Hart, D.J. | ||||||
Citation | Journal: Nat.Struct.Mol.Biol. / Year: 2007 Title: Structure and nuclear import function of the C-terminal domain of influenza virus polymerase PB2 subunit. Authors: Tarendeau, F. / Boudet, J. / Guilligay, D. / Mas, P.J. / Bougault, C.M. / Boulo, S. / Baudin, F. / Ruigrok, R.W. / Daigle, N. / Ellenberg, J. / Cusack, S. / Simorre, J.P. / Hart, D.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2gmo.cif.gz | 237 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2gmo.ent.gz | 194.3 KB | Display | PDB format |
PDBx/mmJSON format | 2gmo.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 2gmo_validation.pdf.gz | 341.9 KB | Display | wwPDB validaton report |
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Full document | 2gmo_full_validation.pdf.gz | 419.9 KB | Display | |
Data in XML | 2gmo_validation.xml.gz | 14.5 KB | Display | |
Data in CIF | 2gmo_validation.cif.gz | 22.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/gm/2gmo ftp://data.pdbj.org/pub/pdb/validation_reports/gm/2gmo | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 8985.251 Da / Num. of mol.: 1 / Fragment: PB2 C-terminal 678-759 fragment Source method: isolated from a genetically manipulated source Source: (gene. exp.) Influenza A virus (A/Victoria/3/1975(H3N2)) Genus: Influenzavirus A / Species: Influenza A virus / Strain: A/Victoria/3/75(H3N2) / Gene: pb2 / Plasmid: pTriEx / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) Codon Plus RIL / References: UniProt: P31345 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample conditions | Ionic strength: 200 mM NaCl / pH: 7.5 / Pressure: ambient / Temperature: 283 K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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-Processing
NMR software |
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Refinement | Method: simulated annealing / Software ordinal: 1 Details: starting from extended conformation, 1000 structures calculated with CNS using distance and dihedral restraints from NOE data and Talos, hydrogen bonds added from exchange experiment | ||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||
NMR ensemble | Conformer selection criteria: 10 structures of lowest energy Conformers calculated total number: 1000 / Conformers submitted total number: 10 |