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- PDB-2flc: Post-Reactive Complex of Restriction Endonuclease HinP1I with Nic... -

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Basic information

Entry
Database: PDB / ID: 2flc
TitlePost-Reactive Complex of Restriction Endonuclease HinP1I with Nicked Cognate DNA and Magnesium Ions
Components
  • 5'-D(*CP*CP*AP*G)-3'
  • 5'-D(*CP*CP*AP*GP*CP*GP*CP*TP*GP*G)-3'
  • 5'-D(P*CP*GP*CP*TP*GP*G)-3'
  • R.HinP1I Restriction Endonuclease
KeywordsHYDROLASE/DNA / RESTRICTION ENDONUCLEASE / PROTEIN DIMERIZATON / DNA SUPERHELIX / PROTEIN-DNA-METAL ION COMPLEX / NICKED DNA / HYDROLASE-DNA COMPLEX
Function / homology
Function and homology information


endonuclease activity / metal ion binding
Similarity search - Function
Trna Endonuclease; Chain: A, domain 1 - #40 / Restriction endonuclease, type II, HinP1I / R.HinP1I restriction endonuclease / Trna Endonuclease; Chain: A, domain 1 / 3-Layer(aba) Sandwich / Alpha Beta
Similarity search - Domain/homology
: / DNA / R.HinP1I restriction endonuclease
Similarity search - Component
Biological speciesHaemophilus influenzae (bacteria)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 2.59 Å
AuthorsHorton, J.R.
Citation
Journal: Nucleic Acids Res. / Year: 2006
Title: DNA nicking by HinP1I endonuclease: bending, base flipping and minor groove expansion.
Authors: Horton, J.R. / Zhang, X. / Maunus, R. / Yang, Z. / Wilson, G.G. / Roberts, R.J. / Cheng, X.
#1: Journal: Nucleic Acids Res. / Year: 2005
Title: Structure of HinP1I endonuclease reveals a striking similarity to the monomeric restriction enzyme MspI
Authors: Yang, Z. / Horton, J.R. / Maunus, R. / Wilson, G.G. / Roberts, R.J. / Cheng, X.
History
DepositionJan 5, 2006Deposition site: RCSB / Processing site: RCSB
Revision 1.0Feb 21, 2006Provider: repository / Type: Initial release
Revision 1.1May 1, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Oct 18, 2017Group: Refinement description / Category: software
Revision 1.4Jan 31, 2018Group: Database references / Category: citation_author / Item: _citation_author.name
Revision 1.5Aug 30, 2023Group: Data collection / Database references ...Data collection / Database references / Derived calculations / Refinement description
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model / pdbx_struct_conn_angle / struct_conn / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_asym_id / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr2_auth_asym_id / _pdbx_struct_conn_angle.ptnr2_auth_seq_id / _pdbx_struct_conn_angle.ptnr2_label_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_asym_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_asym_id / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_asym_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
C: 5'-D(*CP*CP*AP*G)-3'
D: 5'-D(P*CP*GP*CP*TP*GP*G)-3'
E: 5'-D(*CP*CP*AP*GP*CP*GP*CP*TP*GP*G)-3'
A: R.HinP1I Restriction Endonuclease
hetero molecules


Theoretical massNumber of molelcules
Total (without water)34,9588
Polymers34,8394
Non-polymers1204
Water181
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)100.387, 100.387, 139.369
Angle α, β, γ (deg.)90.00, 90.00, 120.00
Int Tables number179
Space group name H-MP6522

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Components

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DNA chain , 3 types, 3 molecules CDE

#1: DNA chain 5'-D(*CP*CP*AP*G)-3'


Mass: 1175.819 Da / Num. of mol.: 1 / Source method: obtained synthetically / Details: Synthesized Self-Annealing Oligonucleotide
#2: DNA chain 5'-D(P*CP*GP*CP*TP*GP*G)-3'


Mass: 1825.216 Da / Num. of mol.: 1 / Source method: obtained synthetically
#3: DNA chain 5'-D(*CP*CP*AP*GP*CP*GP*CP*TP*GP*G)-3'


Mass: 3045.992 Da / Num. of mol.: 1 / Source method: obtained synthetically

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Protein , 1 types, 1 molecules A

#4: Protein R.HinP1I Restriction Endonuclease


Mass: 28791.668 Da / Num. of mol.: 1
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Haemophilus influenzae (bacteria) / Gene: hinP1IR / Plasmid: pUC19 / Production host: Escherichia coli (E. coli) / Strain (production host): PR1
References: GenBank: 57116674, UniProt: Q5I6E6*PLUS, type II site-specific deoxyribonuclease

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Non-polymers , 3 types, 5 molecules

#5: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Mg
#6: Chemical ChemComp-CL / CHLORIDE ION


Mass: 35.453 Da / Num. of mol.: 2 / Source method: obtained synthetically / Formula: Cl
#7: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 2.89 Å3/Da / Density % sol: 57.37 %
Crystal growTemperature: 289 K / Method: vapor diffusion, hanging drop / pH: 6.8
Details: 20% PEG1000, 400mM NaCl, 100mM Bis-Tris propane, and 5% ethylene glycol (5mM Magnesium Chloride was soaked into the crystal), pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 289K
Components of the solutions
IDNameCrystal-IDSol-ID
1PEG100011
2Bis-Tris propane11
3ethylene glycol11
4NaCl11
5H2O11
6PEG100012
7NaCl12
8H2O12

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 22-ID / Wavelength: 1 Å
DetectorType: MARRESEARCH / Detector: CCD / Date: Apr 17, 2005
RadiationMonochromator: Si220 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1 Å / Relative weight: 1
ReflectionResolution: 2.59→34.1 Å / Num. all: 13460 / Num. obs: 13460 / % possible obs: 99.2 % / Observed criterion σ(F): -3 / Observed criterion σ(I): -3 / Redundancy: 7.9 % / Biso Wilson estimate: 56 Å2 / Rmerge(I) obs: 0.07 / Net I/σ(I): 17.6
Reflection shellResolution: 2.59→2.65 Å / Num. unique all: 1059 / % possible all: 95.7

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Processing

Software
NameVersionClassification
MAR345data collection
SCALEPACKdata scaling
GLRFphasing
CNS1.1refinement
RefinementMethod to determine structure: MOLECULAR REPLACEMENT
Starting model: pdb entry 1YNM

1ynm


Resolution: 2.59→34.1 Å / Isotropic thermal model: ISOTROPIC / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
RfactorNum. reflection% reflectionSelection details
Rfree0.324 664 -RANDOM
Rwork0.275 ---
all0.281 13460 --
obs0.28 13068 96.6 %-
Displacement parametersBiso mean: 69.7 Å2
Baniso -1Baniso -2Baniso -3
1-13.81 Å28.58 Å20 Å2
2--13.81 Å20 Å2
3----27.63 Å2
Refine analyze
FreeObs
Luzzati coordinate error0.6 Å0.49 Å
Luzzati d res low-34.1 Å
Luzzati sigma a0.66 Å0.55 Å
Refinement stepCycle: LAST / Resolution: 2.59→34.1 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms1925 405 4 1 2335
Refine LS restraints
Refine-IDTypeDev ideal
X-RAY DIFFRACTIONc_bond_d0.011
X-RAY DIFFRACTIONc_angle_deg1.6
X-RAY DIFFRACTIONc_dihedral_angle_d22.7
X-RAY DIFFRACTIONc_improper_angle_d2.8

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