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- PDB-2fju: Activated Rac1 bound to its effector phospholipase C beta 2 -

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Basic information

Entry
Database: PDB / ID: 2fju
TitleActivated Rac1 bound to its effector phospholipase C beta 2
Components
  • 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta 2
  • Ras-related C3 botulinum toxin substrate 1
KeywordsSIGNALING PROTEIN / APOPTOSIS/HYDROLASE / Protein-protein complex / APOPTOSIS-HYDROLASE COMPLEX
Function / homology
Function and homology information


G-protein beta/gamma-subunit complex / phosphatidylinositol phospholipase C activity / phosphoinositide phospholipase C / regulation of respiratory burst / positive regulation of ovarian follicle development / regulation of neutrophil migration / Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion / negative regulation of interleukin-23 production / Acetylcholine regulates insulin secretion / Activated NTRK2 signals through CDK5 ...G-protein beta/gamma-subunit complex / phosphatidylinositol phospholipase C activity / phosphoinositide phospholipase C / regulation of respiratory burst / positive regulation of ovarian follicle development / regulation of neutrophil migration / Fatty Acids bound to GPR40 (FFAR1) regulate insulin secretion / negative regulation of interleukin-23 production / Acetylcholine regulates insulin secretion / Activated NTRK2 signals through CDK5 / localization within membrane / kinocilium / regulation of cell adhesion involved in heart morphogenesis / ruffle assembly / NTRK2 activates RAC1 / NADPH oxidase complex / phosphatidylinositol metabolic process / Inactivation of CDC42 and RAC1 / engulfment of apoptotic cell / regulation of hydrogen peroxide metabolic process / phosphatidylinositol-4,5-bisphosphate phospholipase C activity / detection of chemical stimulus involved in sensory perception of bitter taste / PLC beta mediated events / WNT5:FZD7-mediated leishmania damping / SEMA3A-Plexin repulsion signaling by inhibiting Integrin adhesion / cortical cytoskeleton organization / cell projection assembly / respiratory burst / C-type glycerophospholipase activity / ruffle organization / midbrain dopaminergic neuron differentiation / positive regulation of bicellular tight junction assembly / GTP-dependent protein binding / negative regulation of fibroblast migration / thioesterase binding / regulation of lamellipodium assembly / regulation of stress fiber assembly / RHO GTPases activate CIT / Nef and signal transduction / motor neuron axon guidance / Activation of RAC1 / PCP/CE pathway / hepatocyte growth factor receptor signaling pathway / sphingosine-1-phosphate receptor signaling pathway / RHO GTPases activate KTN1 / regulation of nitric oxide biosynthetic process / DCC mediated attractive signaling / MET activates RAP1 and RAC1 / Azathioprine ADME / Sema4D mediated inhibition of cell attachment and migration / CD28 dependent Vav1 pathway / Ephrin signaling / positive regulation of neutrophil chemotaxis / Wnt signaling pathway, planar cell polarity pathway / superoxide anion generation / regulation of receptor signaling pathway via JAK-STAT / lamellipodium assembly / NRAGE signals death through JNK / positive regulation of ruffle assembly / forebrain development / small GTPase-mediated signal transduction / Rho GDP-dissociation inhibitor binding / Activation of RAC1 downstream of NMDARs / positive regulation of Rho protein signal transduction / pericentriolar material / Synthesis of IP3 and IP4 in the cytosol / establishment or maintenance of cell polarity / phosphatidylinositol-mediated signaling / semaphorin-plexin signaling pathway / Rac protein signal transduction / RHO GTPases activate PAKs / phospholipid metabolic process / Sema3A PAK dependent Axon repulsion / EPH-ephrin mediated repulsion of cells / ficolin-1-rich granule membrane / positive regulation of focal adhesion assembly / regulation of postsynapse assembly / RHO GTPases Activate NADPH Oxidases / anatomical structure morphogenesis / regulation of synaptic vesicle endocytosis / RHO GTPases Activate WASPs and WAVEs / lipid catabolic process / neuronal dense core vesicle / RHO GTPases activate IQGAPs / RHO GTPases activate PKNs / GPVI-mediated activation cascade / PTK6 Regulates RHO GTPases, RAS GTPase and MAP kinases / positive regulation of lamellipodium assembly / phagocytic cup / release of sequestered calcium ion into cytosol / substrate adhesion-dependent cell spreading / positive regulation of stress fiber assembly / cell projection / RAC1 GTPase cycle / actin filament polymerization / positive regulation of substrate adhesion-dependent cell spreading / EPHB-mediated forward signaling / positive regulation of endothelial cell migration / regulation of cell migration / secretory granule membrane
Similarity search - Function
1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 / : / PH-domain like - #240 / Phosphoinositide-specific phospholipase C, efhand-like / Phospholipase C-beta, C-terminal domain / PLC-beta C terminal / PLC-beta, PH domain / Phospholipase C-beta, C-terminal domain superfamily / : / PH domain ...1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2 / : / PH-domain like - #240 / Phosphoinositide-specific phospholipase C, efhand-like / Phospholipase C-beta, C-terminal domain / PLC-beta C terminal / PLC-beta, PH domain / Phospholipase C-beta, C-terminal domain superfamily / : / PH domain / Phosphoinositide phospholipase C beta1-4-like EF-hand domain / Phosphatidylinositol-4, 5-bisphosphate phosphodiesterase beta / Phosphatidylinositol (PI) phosphodiesterase / Phosphoinositide phospholipase C family / Phospholipase C, phosphatidylinositol-specific, Y domain / Phosphatidylinositol-specific phospholipase C, Y domain / Phosphatidylinositol-specific phospholipase Y-box domain profile. / Phospholipase C, catalytic domain (part); domain Y / Phosphatidylinositol-specific phospholipase C, X domain / Phosphatidylinositol-specific phospholipase C, X domain / Phospholipase C, catalytic domain (part); domain X / Phosphatidylinositol-specific phospholipase X-box domain profile. / PLC-like phosphodiesterase, TIM beta/alpha-barrel domain superfamily / Small GTPase Rho / Small GTPase Rho domain profile. / C2 domain / Protein kinase C conserved region 2 (CalB) / C2 domain / C2 domain / C2 domain profile. / PH-domain like / EF-hand / Recoverin; domain 1 / C2 domain superfamily / Rho (Ras homology) subfamily of Ras-like small GTPases / Ras subfamily of RAS small GTPases / Small GTPase / Ras family / Rab subfamily of small GTPases / EF-hand domain pair / Small GTP-binding protein domain / Roll / P-loop containing nucleotide triphosphate hydrolases / TIM Barrel / Alpha-Beta Barrel / Immunoglobulin-like / Sandwich / Rossmann fold / P-loop containing nucleoside triphosphate hydrolase / Orthogonal Bundle / 3-Layer(aba) Sandwich / Mainly Beta / Mainly Alpha / Alpha Beta
Similarity search - Domain/homology
5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE / Ras-related C3 botulinum toxin substrate 1 / 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase beta-2
Similarity search - Component
Biological speciesHomo sapiens (human)
MethodX-RAY DIFFRACTION / SYNCHROTRON / MIR, MAD, molecular replacement / Resolution: 2.2 Å
AuthorsJezyk, M.R. / Snyder, J.T. / Harden, T.K. / Sondek, J.
CitationJournal: Nat.Struct.Mol.Biol. / Year: 2006
Title: Crystal structure of Rac1 bound to its effector phospholipase C-beta2.
Authors: Jezyk, M.R. / Snyder, J.T. / Gershberg, S. / Worthylake, D.K. / Harden, T.K. / Sondek, J.
History
DepositionJan 3, 2006Deposition site: RCSB / Processing site: RCSB
Revision 1.0Nov 21, 2006Provider: repository / Type: Initial release
Revision 1.1May 1, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Version format compliance
Revision 1.3Feb 14, 2024Group: Data collection / Database references / Derived calculations
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_struct_conn_angle / struct_conn / struct_ref_seq_dif / struct_site
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_struct_conn_angle.ptnr1_auth_comp_id / _pdbx_struct_conn_angle.ptnr1_auth_seq_id / _pdbx_struct_conn_angle.ptnr1_label_asym_id / _pdbx_struct_conn_angle.ptnr1_label_atom_id / _pdbx_struct_conn_angle.ptnr1_label_comp_id / _pdbx_struct_conn_angle.ptnr1_label_seq_id / _pdbx_struct_conn_angle.ptnr3_auth_comp_id / _pdbx_struct_conn_angle.ptnr3_auth_seq_id / _pdbx_struct_conn_angle.ptnr3_label_asym_id / _pdbx_struct_conn_angle.ptnr3_label_atom_id / _pdbx_struct_conn_angle.ptnr3_label_comp_id / _pdbx_struct_conn_angle.ptnr3_label_seq_id / _pdbx_struct_conn_angle.value / _struct_conn.pdbx_dist_value / _struct_conn.ptnr1_auth_comp_id / _struct_conn.ptnr1_auth_seq_id / _struct_conn.ptnr1_label_asym_id / _struct_conn.ptnr1_label_atom_id / _struct_conn.ptnr1_label_comp_id / _struct_conn.ptnr1_label_seq_id / _struct_conn.ptnr2_auth_comp_id / _struct_conn.ptnr2_auth_seq_id / _struct_conn.ptnr2_label_asym_id / _struct_conn.ptnr2_label_atom_id / _struct_conn.ptnr2_label_comp_id / _struct_conn.ptnr2_label_seq_id / _struct_ref_seq_dif.details / _struct_site.pdbx_auth_asym_id / _struct_site.pdbx_auth_comp_id / _struct_site.pdbx_auth_seq_id

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

Downloads & links

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Assembly

Deposited unit
A: Ras-related C3 botulinum toxin substrate 1
B: 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta 2
hetero molecules


Theoretical massNumber of molelcules
Total (without water)111,4355
Polymers110,8322
Non-polymers6043
Water8,755486
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
Unit cell
Length a, b, c (Å)185.822, 185.822, 93.823
Angle α, β, γ (deg.)90.00, 90.00, 120.00
Int Tables number154
Space group name H-MP3221

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Components

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Protein , 2 types, 2 molecules AB

#1: Protein Ras-related C3 botulinum toxin substrate 1 / p21-Rac1 / Ras-like protein TC25


Mass: 19797.844 Da / Num. of mol.: 1 / Fragment: residues 1-189
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: RAC1 / Production host: Escherichia coli (E. coli) / References: UniProt: P63000
#2: Protein 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase beta 2 / E.C.3.1.4.11 / Phosphoinositide phospholipase C / Phospholipase C- beta-2 / PLC-beta-2


Mass: 91033.922 Da / Num. of mol.: 1 / Fragment: residues 1-799
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Homo sapiens (human) / Gene: PLCB2 / Plasmid: pVL1392 / Production host: Spodoptera frugiperda (fall armyworm) / Strain (production host): Sf21
References: UniProt: Q00722, phosphoinositide phospholipase C

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Non-polymers , 4 types, 489 molecules

#3: Chemical ChemComp-MG / MAGNESIUM ION


Mass: 24.305 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Mg
#4: Chemical ChemComp-GSP / 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE


Mass: 539.246 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: C10H16N5O13P3S
#5: Chemical ChemComp-CA / CALCIUM ION


Mass: 40.078 Da / Num. of mol.: 1 / Source method: obtained synthetically / Formula: Ca
#6: Water ChemComp-HOH / water


Mass: 18.015 Da / Num. of mol.: 486 / Source method: isolated from a natural source / Formula: H2O

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Experimental details

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Experiment

ExperimentMethod: X-RAY DIFFRACTION / Number of used crystals: 1

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Sample preparation

CrystalDensity Matthews: 4.22 Å3/Da / Density % sol: 70.83 %
Crystal growTemperature: 291 K / Method: vapor diffusion, sitting drop / pH: 8.5
Details: 8% PEG 3350, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K

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Data collection

DiffractionMean temperature: 100 K
Diffraction sourceSource: SYNCHROTRON / Site: APS / Beamline: 19-ID / Wavelength: 1.009 Å
DetectorType: MARRESEARCH / Detector: CCD / Date: Apr 4, 2004
RadiationMonochromator: Si 220 / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthWavelength: 1.009 Å / Relative weight: 1
ReflectionResolution: 2.2→15 Å / Num. all: 94114 / Num. obs: 93710
Reflection shellResolution: 2.2→2.28 Å

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Processing

Software
NameClassification
HKL-2000data collection
SCALEPACKdata scaling
SHELXmodel building
MLPHAREphasing
DMmodel building
CNSrefinement
HKL-2000data reduction
SHELXphasing
DMphasing
RefinementMethod to determine structure: MIR, MAD, molecular replacement
Resolution: 2.2→15 Å / Cross valid method: THROUGHOUT / σ(F): 0
RfactorNum. reflection% reflectionSelection details
Rfree0.2261 4701 5 %RANDOM
Rwork0.2084 ---
all-94114 --
obs-93710 --
Displacement parametersBiso mean: 32.547 Å2
Refinement stepCycle: LAST / Resolution: 2.2→15 Å
ProteinNucleic acidLigandSolventTotal
Num. atoms6994 0 34 486 7514

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