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Yorodumi- PDB-2ev0: Bacillus subtilis manganese transport regulator (MNTR) bound to c... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2ev0 | ||||||
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Title | Bacillus subtilis manganese transport regulator (MNTR) bound to cadmium | ||||||
Components | Transcriptional regulator mntR | ||||||
Keywords | TRANSCRIPTION / HELIX-TURN-HELIX / DNA-BINDING PROTEIN / METALLOREGULATORY PROTEIN | ||||||
Function / homology | Function and homology information intracellular manganese ion homeostasis / manganese ion binding / protein dimerization activity / DNA-binding transcription factor activity / DNA binding / cytoplasm Similarity search - Function | ||||||
Biological species | Bacillus subtilis (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.65 Å | ||||||
Authors | Kliegman, J.I. / Griner, S.L. / Helmann, J.D. / Brennan, R.G. / Glasfeld, A. | ||||||
Citation | Journal: Biochemistry / Year: 2006 Title: Structural Basis for the Metal-Selective Activation of the Manganese Transport Regulator of Bacillus subtilis. Authors: Kliegman, J.I. / Griner, S.L. / Helmann, J.D. / Brennan, R.G. / Glasfeld, A. | ||||||
History |
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Remark 999 | SEQUENCE THE AUTHOR MAINTAINS THAT THE CORRECT RESIDUE AT THIS LOCATION SHOULD BE GLU. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2ev0.cif.gz | 73.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2ev0.ent.gz | 53.8 KB | Display | PDB format |
PDBx/mmJSON format | 2ev0.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/ev/2ev0 ftp://data.pdbj.org/pub/pdb/validation_reports/ev/2ev0 | HTTPS FTP |
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-Related structure data
Related structure data | 2ev5C 2ev6C 2f5cC 2f5dC 2f5eC 2f5fC 1on1S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 16787.133 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bacillus subtilis (bacteria) / Gene: mntR / Plasmid: PHB7506 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3)-RIL / References: UniProt: P54512 #2: Chemical | ChemComp-CD / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.52 Å3/Da / Density % sol: 51.12 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 8.5 Details: 20% PEG 400, 0.15 M LITHIUM SULFATE, pH 8.50, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: SSRL / Beamline: BL9-2 / Wavelength: 0.98397 |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: Dec 22, 2002 |
Radiation | Monochromator: Graphite / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.98397 Å / Relative weight: 1 |
Reflection | Resolution: 1.65→40 Å / Num. all: 88240 / Num. obs: 37469 / % possible obs: 92.6 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Biso Wilson estimate: 20.5 Å2 / Rmerge(I) obs: 0.048 |
Reflection shell | Resolution: 1.65→1.75 Å / Rmerge(I) obs: 0.195 / % possible all: 90.7 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1ON1 Resolution: 1.65→39.95 Å / Rfactor Rfree error: 0.005 / Data cutoff high absF: 1197629.53 / Data cutoff high rms absF: 1197629.53 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 52.5308 Å2 / ksol: 0.382979 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 28.3 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.65→39.95 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.65→1.75 Å / Rfactor Rfree error: 0.016 / Total num. of bins used: 6
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Xplor file |
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