ascospore-type prospore membrane formation / Golgi to plasma membrane transport vesicle / Anchoring of the basal body to the plasma membrane / RAB GEFs exchange GTP for GDP on RABs / cell tip / cellular bud tip / cellular bud neck / mating projection tip / phosphatidylinositol-4-phosphate binding / sporulation resulting in formation of a cellular spore ...ascospore-type prospore membrane formation / Golgi to plasma membrane transport vesicle / Anchoring of the basal body to the plasma membrane / RAB GEFs exchange GTP for GDP on RABs / cell tip / cellular bud tip / cellular bud neck / mating projection tip / phosphatidylinositol-4-phosphate binding / sporulation resulting in formation of a cellular spore / exocytosis / transport vesicle / guanyl-nucleotide exchange factor activity / autophagy / protein transport / identical protein binding / cytosol 類似検索 - 分子機能
解像度: 3→3.11 Å / 冗長度: 5.6 % / Rmerge(I) obs: 0.422 / Mean I/σ(I) obs: 2.2 / Num. unique all: 6357 / Rsym value: 0.422 / % possible all: 98.2
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解析
ソフトウェア
名称
バージョン
分類
ADSC
Quantum
データ収集
SHARP
位相決定
CNS
1.1
精密化
DENZO
データ削減
SCALEPACK
データスケーリング
精密化
構造決定の手法: 単波長異常分散 / 解像度: 3→50 Å / Isotropic thermal model: Isotropic / 交差検証法: THROUGHOUT / σ(F): 0 / σ(I): 0 / 立体化学のターゲット値: Engh & Huber 詳細: THIS IS A TWINNED STRUCTURE. THE TWINNING OPERATOR IS (H,K,L) -> (h, -k, -l) AND THE TWINNING FRACTION IS 0.500.
Rfactor
反射数
Selection details
Rfree
0.285
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RANDOM
Rwork
0.229
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all
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65695
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obs
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65695
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原子変位パラメータ
Biso mean: 138.1 Å2
Refine analyze
Luzzati coordinate error obs: 0.39 Å / Luzzati sigma a obs: 0.44 Å