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- PDB-2e40: Crystal structure of intracellular family 1 beta-glucosidase BGL1... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2.0E+40 | ||||||
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Title | Crystal structure of intracellular family 1 beta-glucosidase BGL1A from the basidiomycete Phanerochaete chrysosporium in complex with gluconolactone | ||||||
![]() | Beta-glucosidase | ||||||
![]() | HYDROLASE / TIM BARREL / GLYCOSIDE HYDROLASE FAMILY 1 / CLAN GH-A / Structural Genomics / NPPSFA / National Project on Protein Structural and Functional Analyses | ||||||
Function / homology | ![]() cellobiose glucosidase activity / scopolin beta-glucosidase activity / beta-glucosidase / beta-glucosidase activity / cellulose catabolic process / cytosol Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Nijikken, Y. / Tsukada, T. / Igarashi, K. / Samejima, M. / Fushinobu, S. | ||||||
![]() | ![]() Title: Crystal structure of intracellular family 1 beta-glucosidase BGL1A from the basidiomycete Phanerochaete chrysosporium Authors: Nijikken, Y. / Tsukada, T. / Igarashi, K. / Samejima, M. / Wakagi, T. / Shoun, H. / Fushinobu, S. #1: Journal: Appl.Microbiol.Biotechnol. / Year: 2006 Title: Molecular cloning and characterization of two intracellular beta-glucosidases belonging to glycoside hydrolase family 1 from the basidiomycete Phanerochaete chrysosporium Authors: Tsukada, T. / Igarashi, K. / Yoshida, M. / Samejima, M. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 214.5 KB | Display | ![]() |
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PDB format | ![]() | 168.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 446.4 KB | Display | ![]() |
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Full document | ![]() | 453.9 KB | Display | |
Data in XML | ![]() | 42.2 KB | Display | |
Data in CIF | ![]() | 64.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2e3zC ![]() 1cbgS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Details | The biological assembly is a monomer. The asymmetric unit contains two almost identical monomers. |
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Components
#1: Protein | Mass: 52959.008 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Sugar | #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.48 Å3/Da / Density % sol: 50.4 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 5.8 Details: 15% PEG 6000, 12% isopropanol, 0.1M sodium citrate, pH 5.8, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Dec 5, 2004 |
Radiation | Monochromator: Triangular Si(111) with an asymmetric angle of 7.8 deg Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→50 Å / Num. obs: 83722 / % possible obs: 99.5 % / Observed criterion σ(I): 0 / Redundancy: 4.2 % / Biso Wilson estimate: 15.5 Å2 / Rmerge(I) obs: 0.078 / Net I/σ(I): 19.4 |
Reflection shell | Resolution: 1.9→1.97 Å / Rmerge(I) obs: 0.286 / Mean I/σ(I) obs: 3.59 / % possible all: 99.4 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1CBG Resolution: 1.9→44.57 Å / Rfactor Rfree error: 0.003 / Data cutoff high absF: 2484620.95 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 45.7929 Å2 / ksol: 0.361751 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 21.5 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.9→44.57 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.9→2.02 Å / Rfactor Rfree error: 0.009 / Total num. of bins used: 6
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Xplor file |
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