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- PDB-1cbg: THE CRYSTAL STRUCTURE OF A CYANOGENIC BETA-GLUCOSIDASE FROM WHITE... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1cbg | ||||||
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Title | THE CRYSTAL STRUCTURE OF A CYANOGENIC BETA-GLUCOSIDASE FROM WHITE CLOVER (TRIFOLIUM REPENS L.), A FAMILY 1 GLYCOSYL-HYDROLASE | ||||||
![]() | CYANOGENIC BETA-GLUCOSIDASE | ||||||
![]() | HYDROLASE (O-GLYCOSYL) / CYANOGENIC BETA-GLUCOSIDASE | ||||||
Function / homology | ![]() scopolin beta-glucosidase activity / beta-glucosidase / beta-glucosidase activity / carbohydrate metabolic process Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Barrett, T.E. / Suresh, C.G. / Tolley, S.P. / Hughes, M.A. | ||||||
![]() | ![]() Title: The crystal structure of a cyanogenic beta-glucosidase from white clover, a family 1 glycosyl hydrolase. Authors: Barrett, T. / Suresh, C.G. / Tolley, S.P. / Dodson, E.J. / Hughes, M.A. #1: ![]() Title: Crystallization and Preliminary Crystallographic Analysis of the Cyanogenic Beta-Glucosidase from the White Clover Trifolium Repens L Authors: Tolley, S.P. / Barrett, T.E. / Suresh, C.G. / Hughes, M.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 116 KB | Display | ![]() |
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PDB format | ![]() | 94 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 367 KB | Display | ![]() |
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Full document | ![]() | 378.5 KB | Display | |
Data in XML | ![]() | 12 KB | Display | |
Data in CIF | ![]() | 20.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO 199 2: TRP 446 - SER 447 OMEGA = 358.47 PEPTIDE BOND DEVIATES SIGNIFICANTLY FROM TRANS CONFORMATION | ||||||||
Details | SYMMETRY THE CRYSTALLOGRAPHIC SYMMETRY TRANSFORMATIONS PRESENTED BELOW GENERATE THE SUBUNITS OF THE POLYMERIC MOLECULE. APPLIED TO RESIDUES: PHE 1 .. LYS 490 THE CYANOGENIC BETA-GLUCOSIDASE FROM WHITE CLOVER EXISTS AS A HOMODIMER IN SOLUTION. THE DIMERS ARE RELATED BY THE CRYSTALLOGRAPHIC TWO-FOLD AXIS PASSING THROUGH THE UNIT CELL ORIGIN IN THE AB PLANE. SYMMETRY1 1 0.000000 -1.000000 0.000000 1.00000 SYMMETRY2 1 -1.000000 0.000000 0.000000 1.00000 SYMMETRY3 1 0.000000 0.000000 -1.000000 0.50000 |
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Components
#1: Protein | Mass: 56419.664 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.73 Å3/Da / Density % sol: 54.91 % | |||||||||||||||
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Crystal grow | pH: 6 / Details: pH 6.0 | |||||||||||||||
Crystal grow | *PLUS Temperature: 20 ℃ / pH: 6.2 / Method: vapor diffusion, hanging drop / Details: Tolley, S.P., (1993) J.Mol.Biol., 229, 791. | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Apr 16, 1994 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.88 Å / Relative weight: 1 |
Reflection | Resolution: 2.15→15 Å / Num. obs: 30663 / % possible obs: 88 % / Observed criterion σ(I): 3.5 / Redundancy: 3 % / Rmerge(I) obs: 0.057 |
Reflection | *PLUS Rmerge(I) obs: 0.057 |
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Processing
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Refinement | Resolution: 2.15→9 Å / σ(F): 0 Details: WATER MOLECULES WITH OCCUPANCIES OF 0.5 HAVE BEEN ASSIGNED TO DENSITY THAT IS PROBABLY ATTRIBUTABLE TO GLYCOSYLATION (IN THE VICINITY OF RESIDUES ASN 15 AND ASN 315) BUT IS OF INSUFFICIENT ...Details: WATER MOLECULES WITH OCCUPANCIES OF 0.5 HAVE BEEN ASSIGNED TO DENSITY THAT IS PROBABLY ATTRIBUTABLE TO GLYCOSYLATION (IN THE VICINITY OF RESIDUES ASN 15 AND ASN 315) BUT IS OF INSUFFICIENT QUALITY TO MODEL AS SUGAR RESIDUES.
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Displacement parameters | Biso mean: 12 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.15→9 Å
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Refine LS restraints |
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Refinement | *PLUS Rfactor obs: 0.189 / Rfactor Rwork: 0.189 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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