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- PDB-2dhr: Whole cytosolic region of ATP-dependent metalloprotease FtsH (G399L) -
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Open data
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Basic information
Entry | Database: PDB / ID: 2dhr | ||||||
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Title | Whole cytosolic region of ATP-dependent metalloprotease FtsH (G399L) | ||||||
![]() | FtsH | ||||||
![]() | HYDROLASE / AAA+ protein / hexameric Zn metalloprotease | ||||||
Function / homology | ![]() Hydrolases; Acting on peptide bonds (peptidases); Metalloendopeptidases / ATP-dependent peptidase activity / protein catabolic process / metalloendopeptidase activity / ATP hydrolysis activity / proteolysis / zinc ion binding / ATP binding / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Suno, R. / Niwa, H. / Tsuchiya, D. / Zhang, X. / Yoshida, M. / Morikawa, K. | ||||||
![]() | ![]() Title: Structure of the Whole Cytosolic Region of ATP-Dependent Protease FtsH Authors: Suno, R. / Niwa, H. / Tsuchiya, D. / Zhang, X. / Yoshida, M. / Morikawa, K. #1: ![]() Title: Hexameric ring structure of the ATPase domain of the membrane-integrated metalloprotease FtsH from Thermus thermophilus HB8 Authors: Niwa, H. / Tsuchiya, D. / Makyio, H. / Yoshida, M. / Morikawa, K. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 521.7 KB | Display | ![]() |
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PDB format | ![]() | 425.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.6 MB | Display | ![]() |
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Full document | ![]() | 1.8 MB | Display | |
Data in XML | ![]() | 114.6 KB | Display | |
Data in CIF | ![]() | 151.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Details | The biological assembly is a hexamer composed of the six polypeptide chains in the asymmetric unit. |
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Components
#1: Protein | Mass: 55369.281 Da / Num. of mol.: 6 / Fragment: whole cytosolic region / Mutation: G399L Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Chemical | ChemComp-ADP / |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.24 Å3/Da / Density % sol: 62.01 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7.2 Details: 0.1M Tris-HCl, 1% PEG8K, 0.2mM Zn acetate, 2.5mM ADP, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 315 / Detector: CCD / Date: May 20, 2005 |
Radiation | Monochromator: Si / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 3.9→100 Å / Num. all: 40304 / Num. obs: 40304 / % possible obs: 99.9 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.077 |
Reflection shell | Resolution: 3.9→4.1 Å / Rmerge(I) obs: 0.547 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]()
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Refinement step | Cycle: LAST / Resolution: 3.9→15 Å
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