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Open data
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Basic information
Entry | Database: PDB / ID: 2cbp | |||||||||
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Title | CUCUMBER BASIC PROTEIN, A BLUE COPPER PROTEIN | |||||||||
![]() | CUCUMBER BASIC PROTEIN | |||||||||
![]() | ELECTRON TRANSPORT / PHYTOCYANIN / TYPE 1 COPPER PROTEIN | |||||||||
Function / homology | ![]() pollination / anther development / apoplast / extracellular matrix / electron transfer activity / metal ion binding Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Guss, J.M. / Freeman, H.C. | |||||||||
![]() | ![]() Title: The structure of a phytocyanin, the basic blue protein from cucumber, refined at 1.8 A resolution. Authors: Guss, J.M. / Merritt, E.A. / Phizackerley, R.P. / Freeman, H.C. #1: ![]() Title: Phase Determination by Multiple-Wavelength X-Ray Diffraction. Crystal Structure of a Basic 'Blue' Copper Protein from Cucumbers Authors: Guss, J.M. / Merritt, E.A. / Phizackerley, R.P. / Hedman, B. / Murata, M. / Hodgson, K.O. / Freeman, H.C. #2: ![]() Title: Preliminary Crystallographic Data for a Basic Copper-Containing Protein from Cucumber Seedlings Authors: Colman, P.M. / Freeman, H.C. / Guss, J.M. / Murata, M. / Norris, V.A. / Ramshaw, J.A. / Venkatappa, M.P. / Vickery, L.E. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 51.2 KB | Display | ![]() |
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PDB format | ![]() | 37.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 369.4 KB | Display | ![]() |
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Full document | ![]() | 370.4 KB | Display | |
Data in XML | ![]() | 3.7 KB | Display | |
Data in CIF | ![]() | 5.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 10371.791 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: FROM SEEDLINGS / Source: (natural) ![]() |
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#2: Chemical | ChemComp-CU / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.26 Å3/Da / Density % sol: 47 % | |||||||||||||||
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Crystal grow | *PLUS pH: 6 / Method: vapor diffusion, hanging drop | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Source: ![]() ![]() ![]() | |||||||||||||||
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Detector | Type: XUONG-HAMLIN MULTIWIRE / Detector: AREA DETECTOR / Date: 1986 | |||||||||||||||
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray | |||||||||||||||
Radiation wavelength |
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Reflection | Num. obs: 43466 / % possible obs: 89 % / Observed criterion σ(I): 1 / Rmerge(I) obs: 0.043 |
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Processing
Software | Name: PROLSQ / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Resolution: 1.8→7 Å / σ(F): 0.5 Details: 1988 ESTIMATED COORD. ERROR 0.12 ANGSTROMS (BACKBONE) ESTIMATED COORD. ERROR 0.17 ANGSTROMS (SIDE CHAIN)
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Displacement parameters | Biso mean: 11.9 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.15 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→7 Å
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Refine LS restraints |
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Refinement | *PLUS Rfactor obs: 0.141 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |