POLYMERASE / P2 DNA POLYMERASE IV / 7 / 8-DIHYDRO-8-OXODEOXYGUANOSINE / TRANSLESION DNA POLYMERASE / NUCLEOTIDYLTRANSFERASE / TRANSFERASE / DNA DAMAGE / DNA REPAIR / DNA REPLICATION / DNA- BINDING / DNA-DIRECTED DNA POLYMERASE / MAGNESIUM / METAL - BINDING / MUTATOR PROTEIN
機能・相同性
機能・相同性情報
error-prone translesion synthesis / DNA-templated DNA replication / damaged DNA binding / DNA-directed DNA polymerase / DNA-directed DNA polymerase activity / magnesium ion binding / cytoplasm 類似検索 - 分子機能
DNA polymerase type-Y, HhH motif / IMS family HHH motif / : / DNA polymerase IV / DNA polymerase, Y-family, little finger domain / MutS, DNA mismatch repair protein, domain I - #60 / MutS, DNA mismatch repair protein, domain I / DNA polymerase, Y-family, little finger domain / impB/mucB/samB family C-terminal domain / UmuC domain ...DNA polymerase type-Y, HhH motif / IMS family HHH motif / : / DNA polymerase IV / DNA polymerase, Y-family, little finger domain / MutS, DNA mismatch repair protein, domain I - #60 / MutS, DNA mismatch repair protein, domain I / DNA polymerase, Y-family, little finger domain / impB/mucB/samB family C-terminal domain / UmuC domain / DNA polymerase, Y-family, little finger domain superfamily / impB/mucB/samB family / UmuC domain profile. / Reverse transcriptase/Diguanylate cyclase domain / Dna Ligase; domain 1 / 5' to 3' exonuclease, C-terminal subdomain / DNA polymerase; domain 1 / Reverse transcriptase/Diguanylate cyclase domain / Alpha-Beta Plaits / DNA/RNA polymerase superfamily / 2-Layer Sandwich / Orthogonal Bundle / 3-Layer(aba) Sandwich / Mainly Alpha / Alpha Beta 類似検索 - ドメイン・相同性
2'-DEOXYGUANOSINE-5'-TRIPHOSPHATE / DNA / DNA (> 10) / DNA polymerase IV 類似検索 - 構成要素
マシュー密度: 2.62 Å3/Da / 溶媒含有率: 51.85 % 解説: THE STARTING MODEL WAS THE PDB FILE WITH THE ACCESSION CODE 2BR0. ITS POSITION WAS OPTIMIZED BY SEVERAL ROUNDS OF RIGID BODY REFINEMENT.
結晶化
pH: 7.5 詳細: 5% PEG 3350, 10 MM TRIS-HCL PH 7.5, 50 MM CA(CH3CO2)2, 30MM NACL, 1.25% GLYCEROL
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データ収集
回折
平均測定温度: 110 K
放射光源
由来: シンクロトロン / サイト: APS / ビームライン: 22-ID / 波長: 0.98
検出器
タイプ: MARRESEARCH / 検出器: CCD / 日付: 2005年8月1日
放射
プロトコル: SINGLE WAVELENGTH / 単色(M)・ラウエ(L): M / 散乱光タイプ: x-ray
放射波長
波長: 0.98 Å / 相対比: 1
反射
解像度: 2.56→32.47 Å / Num. obs: 17101 / % possible obs: 99.8 % / Observed criterion σ(I): 1 / 冗長度: 6.78 % / Biso Wilson estimate: 57.9 Å2 / Rmerge(I) obs: 0.07 / Net I/σ(I): 7.9
解像度: 2.56→32.47 Å / Rfactor Rfree error: 0.009 / Data cutoff high absF: 967809.56 / Isotropic thermal model: RESTRAINED / 交差検証法: THROUGHOUT / σ(F): 0 詳細: THE FIRST TEMPLATE NUCLEOTIDE IS DISORDERED AND IS MISSING FROM THE STRUCTURE