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Yorodumi- PDB-1n56: Y-family DNA polymerase Dpo4 in complex with DNA containing abasi... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1n56 | ||||||
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Title | Y-family DNA polymerase Dpo4 in complex with DNA containing abasic lesion | ||||||
Components |
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Keywords | TRANSFERASE/DNA / Y-family / DNA polymerase / DNA lesion bypass / protein-DNA complex / TRANSFERASE-DNA COMPLEX | ||||||
Function / homology | Function and homology information error-prone translesion synthesis / DNA-templated DNA replication / damaged DNA binding / DNA-directed DNA polymerase / DNA-directed DNA polymerase activity / magnesium ion binding / cytoplasm Similarity search - Function | ||||||
Biological species | Sulfolobus solfataricus (archaea) synthetic construct (others) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.4 Å | ||||||
Authors | Ling, H. / Boudsocq, F. / Woodgate, R. / Yang, W. | ||||||
Citation | Journal: Mol.Cell / Year: 2004 Title: Snapshots of replication through an abasic lesion; structural basis for base substitutions and frameshifts. Authors: Ling, H. / Boudsocq, F. / Woodgate, R. / Yang, W. #1: Journal: Cell(Cambridge,Mass.) / Year: 2001 Title: Crystal structure of a Y-familyDNA polymerase in action: a mechanism for error-prone and lesion-bypass replication Authors: Ling, H. / Boudsocq, F. / Woodgate, R. / Yang, W. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1n56.cif.gz | 197.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1n56.ent.gz | 151.6 KB | Display | PDB format |
PDBx/mmJSON format | 1n56.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1n56_validation.pdf.gz | 1.2 MB | Display | wwPDB validaton report |
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Full document | 1n56_full_validation.pdf.gz | 1.2 MB | Display | |
Data in XML | 1n56_validation.xml.gz | 39.5 KB | Display | |
Data in CIF | 1n56_validation.cif.gz | 56 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/n5/1n56 ftp://data.pdbj.org/pub/pdb/validation_reports/n5/1n56 | HTTPS FTP |
-Related structure data
Related structure data | 1s0nC 1s0oC 1s10C 1jx4S S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
-DNA chain , 2 types, 4 molecules CEDF
#1: DNA chain | Mass: 4418.892 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: Template / Source: (synth.) synthetic construct (others) #2: DNA chain | Mass: 4925.175 Da / Num. of mol.: 2 / Source method: obtained synthetically / Details: primer / Source: (synth.) synthetic construct (others) |
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-Protein , 1 types, 2 molecules AB
#3: Protein | Mass: 40257.879 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Sulfolobus solfataricus (archaea) / Gene: dpo4 / Plasmid: pet22bB / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: Q97W02 |
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-Non-polymers , 4 types, 503 molecules
#4: Chemical | #5: Chemical | #6: Chemical | ChemComp-CA / | #7: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.65 Å3/Da / Density % sol: 53.59 % | ||||||||||||||||||||||||||||||||||||
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, hanging drop / pH: 7 Details: 12% PEG 3350, 0.1M Hepes, 100mM calcium acetate, 2.5% glycerol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K | ||||||||||||||||||||||||||||||||||||
Components of the solutions |
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-Data collection
Diffraction | Mean temperature: 95 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU200HB / Wavelength: 1.5418 Å |
Detector | Type: RIGAKU RAXIS II / Detector: IMAGE PLATE / Date: Oct 15, 2001 / Details: mirrors |
Radiation | Monochromator: YALE MIRRORS / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.4→29.9 Å / Num. all: 42232 / Num. obs: 39503 / % possible obs: 93.5 % / Observed criterion σ(F): 0 / Observed criterion σ(I): -2 / Biso Wilson estimate: 64.4 Å2 / Rmerge(I) obs: 0.038 / Rsym value: 0.038 |
Reflection shell | Resolution: 2.4→2.44 Å / Rmerge(I) obs: 0.399 / % possible all: 80 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 1JX4 Resolution: 2.4→29.9 Å / Isotropic thermal model: Isotropic / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber
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Displacement parameters | Biso mean: 66.7 Å2 | |||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.4→29.9 Å
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Refine LS restraints |
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