#200 - Aug 2016 Quasisymmetry in Icosahedral Viruses similarity (1)
-
Assembly
Deposited unit
A: COAT PROTEIN B: COAT PROTEIN C: COAT PROTEIN R: 5'-R(*AP*CP*AP*UP*GP*AP*GP*GP*AP*UP *UP*AP*CP*CP*CP*AP*UP*GP*U)-3' S: 5'-R(*AP*CP*AP*UP*GP*AP*GP*GP*AP*UP *UP*AP*CP*CP*CP*AP*UP*GP*U)-3'
A: COAT PROTEIN B: COAT PROTEIN C: COAT PROTEIN R: 5'-R(*AP*CP*AP*UP*GP*AP*GP*GP*AP*UP *UP*AP*CP*CP*CP*AP*UP*GP*U)-3' S: 5'-R(*AP*CP*AP*UP*GP*AP*GP*GP*AP*UP *UP*AP*CP*CP*CP*AP*UP*GP*U)-3'
A: COAT PROTEIN B: COAT PROTEIN C: COAT PROTEIN R: 5'-R(*AP*CP*AP*UP*GP*AP*GP*GP*AP*UP *UP*AP*CP*CP*CP*AP*UP*GP*U)-3' S: 5'-R(*AP*CP*AP*UP*GP*AP*GP*GP*AP*UP *UP*AP*CP*CP*CP*AP*UP*GP*U)-3'
x 5
icosahedral pentamer
266 kDa, 25 polymers
Theoretical mass
Number of molelcules
Total (without water)
266,059
25
Polymers
266,059
25
Non-polymers
0
0
Water
0
Type
Name
Symmetry operation
Number
identity operation
1_555
x,y,z
1
point symmetry operation
4
4
A: COAT PROTEIN B: COAT PROTEIN C: COAT PROTEIN R: 5'-R(*AP*CP*AP*UP*GP*AP*GP*GP*AP*UP *UP*AP*CP*CP*CP*AP*UP*GP*U)-3' S: 5'-R(*AP*CP*AP*UP*GP*AP*GP*GP*AP*UP *UP*AP*CP*CP*CP*AP*UP*GP*U)-3'
x 6
icosahedral 23 hexamer
319 kDa, 30 polymers
Theoretical mass
Number of molelcules
Total (without water)
319,271
30
Polymers
319,271
30
Non-polymers
0
0
Water
0
Type
Name
Symmetry operation
Number
identity operation
1_555
x,y,z
1
point symmetry operation
5
5
Idetical with deposited unit in distinct coordinate
icosahedral asymmetric unit, std point frame
Type
Name
Symmetry operation
Number
transform to point frame
1
6
A: COAT PROTEIN B: COAT PROTEIN C: COAT PROTEIN R: 5'-R(*AP*CP*AP*UP*GP*AP*GP*GP*AP*UP *UP*AP*CP*CP*CP*AP*UP*GP*U)-3' S: 5'-R(*AP*CP*AP*UP*GP*AP*GP*GP*AP*UP *UP*AP*CP*CP*CP*AP*UP*GP*U)-3'
x 30
crystal asymmetric unit, crystal frame
1.6 MDa, 150 polymers
Theoretical mass
Number of molelcules
Total (without water)
1,596,355
150
Polymers
1,596,355
150
Non-polymers
0
0
Water
0
Type
Name
Symmetry operation
Number
identity operation
1_555
x,y,z
2
point symmetry operation
29
Unit cell
Length a, b, c (Å)
467.050, 285.590, 273.300
Angle α, β, γ (deg.)
90.00, 122.23, 90.00
Int Tables number
5
Space group name H-M
C121
Symmetry
Point symmetry: (Schoenflies symbol: I (icosahedral))
Mass: 6062.657 Da / Num. of mol.: 2 / Source method: obtained synthetically / Source: (synth.) BACTERIOPHAGE MS2 (virus)
Compound details
ENGINEERED RESIDUE IN CHAIN A, ASN 87 TO ALA ENGINEERED RESIDUE IN CHAIN A, GLU 89 TO LYS ...ENGINEERED RESIDUE IN CHAIN A, ASN 87 TO ALA ENGINEERED RESIDUE IN CHAIN A, GLU 89 TO LYS ENGINEERED RESIDUE IN CHAIN B, ASN 87 TO ALA ENGINEERED RESIDUE IN CHAIN B, GLU 89 TO LYS ENGINEERED RESIDUE IN CHAIN C, ASN 87 TO ALA ENGINEERED RESIDUE IN CHAIN C, GLU 89 TO LYS
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Experimental details
-
Experiment
Experiment
Method: X-RAY DIFFRACTION / Number of used crystals: 1
-
Sample preparation
Crystal
Description: NONE
-
Data collection
Diffraction
Mean temperature: 100 K
Diffraction source
Source: SYNCHROTRON / Site: MAX II / Beamline: I711 / Wavelength: 1.1
Detector
Type: MARRESEARCH / Detector: CCD / Date: Feb 1, 2003
Radiation
Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelength
Wavelength: 1.1 Å / Relative weight: 1
Reflection
Resolution: 2.9→30 Å / Num. obs: 221184 / % possible obs: 33 % / Observed criterion σ(I): 0 / Redundancy: 1.5 % / Biso Wilson estimate: 33.5 Å2 / Rmerge(I) obs: 0.2 / Net I/σ(I): 7.2
Reflection shell
Resolution: 2.9→2.95 Å / Redundancy: 1 % / Rmerge(I) obs: 0.5 / Mean I/σ(I) obs: 1.4 / % possible all: 7
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