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- PDB-2b2g: MS2 Wild-type RNA stemloop complexed with an N87S mutant MS2 capsid -
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Open data
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Basic information
Entry | Database: PDB / ID: 2b2g | ||||||
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Title | MS2 Wild-type RNA stemloop complexed with an N87S mutant MS2 capsid | ||||||
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![]() | VIRUS/VIRAL PROTEIN/RNA / CAPSID / COMPLEX (CAPSID PROTEIN - RNA HAIRPIN) / HAIRPIN / LEVIVIRUS / VIRUS/VIRAL / PROTEIN/RNA / VIRUS-VIRAL PROTEIN-RNA COMPLEX | ||||||
Function / homology | ![]() negative regulation of viral translation / T=3 icosahedral viral capsid / regulation of translation / structural molecule activity / RNA binding / identical protein binding Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Horn, W.T. / Tars, K. / Grahn, E. / Helgstrand, C. / Baron, A.J. / Lago, H. / Adams, C.J. / Peabody, D.S. / Phillips, S.E.V. / Stonehouse, N.J. ...Horn, W.T. / Tars, K. / Grahn, E. / Helgstrand, C. / Baron, A.J. / Lago, H. / Adams, C.J. / Peabody, D.S. / Phillips, S.E.V. / Stonehouse, N.J. / Liljas, L. / Stockley, P.G. | ||||||
![]() | ![]() Title: Structural basis of RNA binding discrimination between bacteriophages Qbeta and MS2 Authors: Horn, W.T. / Tars, K. / Grahn, E. / Helgstrand, C. / Baron, A.J. / Lago, H. / Adams, C.J. / Peabody, D.S. / Phillips, S.E.V. / Stonehouse, N.J. / Liljas, L. / Stockley, P.G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 109.7 KB | Display | ![]() |
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PDB format | ![]() | 81.6 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
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-Validation report
Summary document | ![]() | 409.3 KB | Display | ![]() |
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Full document | ![]() | 428.9 KB | Display | |
Data in XML | ![]() | 11.5 KB | Display | |
Data in CIF | ![]() | 18.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1zseC ![]() 2b2dC ![]() 2b2eC ![]() 2bnyC ![]() 2bq5C ![]() 2bs0C ![]() 2bs1C ![]() 2ms2S C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Symmetry | Point symmetry: (Hermann–Mauguin notation: 532 / Schoenflies symbol: I (icosahedral)) | |||||||||||||||||||||||||||||||||
Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: RNA chain | Mass: 6062.657 Da / Num. of mol.: 2 / Source method: obtained synthetically #2: Protein | Mass: 13711.438 Da / Num. of mol.: 3 / Mutation: N87S Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal grow | Temperature: 303 K / Method: vapor diffusion, hanging drop / pH: 7.4 Details: MS2 COATPROTEIN in 1.25% or 1.5% PEG 8000, 0.4M SODIUM PHOSPHATE BUFFER, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 303K | ||||||||||||||||||||||||||||
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Components of the solutions |
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-Data collection
Diffraction | Mean temperature: 298 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Nov 26, 2001 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.488 Å / Relative weight: 1 |
Reflection | Resolution: 3→30 Å / Num. all: 201426 / Num. obs: 162584 / % possible obs: 80.7 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 1.6 % / Rsym value: 0.193 / Net I/σ(I): 8.2 |
Reflection shell | Resolution: 3.02→3.16 Å / Redundancy: 1 % / Mean I/σ(I) obs: 1.9 / Rsym value: 0.342 / % possible all: 44.4 |
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Processing
Software |
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 2MS2 Resolution: 3.02→30 Å / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 0 / Stereochemistry target values: Engh & Huber Details: The waters listed in remark 525 are ncs symmetry related waters which are in hydrogen bonding distance to protein or RNA when the biological molecule is generated. NCS symmetry related B ...Details: The waters listed in remark 525 are ncs symmetry related waters which are in hydrogen bonding distance to protein or RNA when the biological molecule is generated. NCS symmetry related B chain instead of the chain used for the actual refinement has been included so that the protein-RNA interactions between the AB dimer and the R chain RNA are clear without generating the NCS symmetry related 'B' chain.
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 3.02→30 Å
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Refine LS restraints |
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