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- PDB-1mst: CRYSTAL STRUCTURE OF MS2 CAPSIDS WITH MUTATIONS IN THE SUBUNIT FG LOOP -
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Open data
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Basic information
Entry | Database: PDB / ID: 1mst | |||||||||
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Title | CRYSTAL STRUCTURE OF MS2 CAPSIDS WITH MUTATIONS IN THE SUBUNIT FG LOOP | |||||||||
![]() | BACTERIOPHAGE MS2 CAPSID | |||||||||
![]() | VIRUS / BACTERIOPHAGE COAT PROTEIN / Icosahedral virus | |||||||||
Function / homology | ![]() negative regulation of viral translation / T=3 icosahedral viral capsid / regulation of translation / structural molecule activity / RNA binding / identical protein binding Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Liljas, L. / Stonehouse, N.J. | |||||||||
![]() | ![]() Title: Crystal structures of MS2 capsids with mutations in the subunit FG loop. Authors: Stonehouse, N.J. / Valegard, K. / Golmohammadi, R. / van den Worm, S. / Walton, C. / Stockley, P.G. / Liljas, L. #1: ![]() Title: Structure Determination of the Bacteriophage MS2 Authors: Valegard, K. / Liljas, L. / Fridborg, K. / Unge, T. #2: ![]() Title: The Three-Dimensional Structure of the Bacterial Virus MS2 Authors: Valegard, K. / Liljas, L. / Fridborg, K. / Unge, T. #3: ![]() Title: The Structure of Bacteriophage MS2 Authors: Liljas, L. / Valegard, K. #4: ![]() Title: Purification, Crystallization and Preliminary X-Ray Data of the Bacteriophage MS2 Authors: Valegard, K. / Unge, T. / Montelius, I. / Strandberg, B. / Fiers, W. | |||||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 87.8 KB | Display | ![]() |
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PDB format | ![]() | 68 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 376.2 KB | Display | ![]() |
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Full document | ![]() | 380.2 KB | Display | |
Data in XML | ![]() | 8.1 KB | Display | |
Data in CIF | ![]() | 14.2 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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2 |
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3 | ![]()
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4 | ![]()
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5 | ![]()
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6 | ![]()
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO B 78 | |||||||||||||||||||||||||||||||||
Symmetry | Point symmetry: (Hermann–Mauguin notation: 532 / Schoenflies symbol: I (icosahedral)) | |||||||||||||||||||||||||||||||||
Noncrystallographic symmetry (NCS) | NCS oper:
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Components
#1: Protein | Mass: 13724.438 Da / Num. of mol.: 3 / Mutation: E76D Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal grow | *PLUS Temperature: 37 ℃ / pH: 7.4 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||||||||
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Components of the solutions | *PLUS
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-Data collection
Diffraction source | Source: ![]() ![]() ![]() | ||||||||||||
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Detector | Type: MARRESEARCH / Detector: IMAGE PLATE | ||||||||||||
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray | ||||||||||||
Radiation wavelength |
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Reflection | Num. obs: 247689 / Rmerge(I) obs: 0.115 | ||||||||||||
Reflection | *PLUS Highest resolution: 2.6 Å / Lowest resolution: 10.59 Å / % possible obs: 79 % / Rmerge(I) obs: 0.115 | ||||||||||||
Reflection shell | *PLUS Highest resolution: 2.6 Å / Lowest resolution: 2.67 Å / % possible obs: 47 % / Num. unique obs: 10886 / Rmerge(I) obs: 0.313 |
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Processing
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Refinement | Resolution: 2.6→15 Å / σ(F): 2 /
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Refinement step | Cycle: LAST / Resolution: 2.6→15 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS |