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- PDB-2boe: Catalytic domain of endo-1,4-glucanase Cel6A mutant Y73S from The... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2boe | ||||||
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Title | Catalytic domain of endo-1,4-glucanase Cel6A mutant Y73S from Thermobifida fusca | ||||||
![]() | ENDOGLUCANASE E-2 | ||||||
![]() | HYDROLASE / ENDOGLUCANASE / THERMOBIFIDA FUSCA / TIM A/B FOLD / GLYCOSIDE HYDROLASE FAMILY 6 / METHYL CELLOBIOSYL-4-THIO-BETA-CELLOBIOSIDE / CARBOHYDRATE METABOLISM / CELLULOSE DEGRADATION / GLYCOSIDASE / POLYSACCHARIDE DEGRADATION | ||||||
Function / homology | ![]() cellulase / polysaccharide binding / cellulase activity / cellulose catabolic process Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Larsson, A.M. / Bergfors, T. / Dultz, E. / Irwin, D.C. / Roos, A. / Driguez, H. / Wilson, D.B. / Jones, T.A. | ||||||
![]() | ![]() Title: Crystal Structure of Thermobifida Fusca Endoglucanase Cel6A in Complex with Substrate and Inhibitor: The Role of Tyrosine Y73 in Substrate Ring Distortion. Authors: Larsson, A.M. / Bergfors, T. / Dultz, E. / Irwin, D.C. / Roos, A. / Driguez, H. / Wilson, D.B. / Jones, T.A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 134.3 KB | Display | ![]() |
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PDB format | ![]() | 104.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 412 KB | Display | ![]() |
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Full document | ![]() | 414.9 KB | Display | |
Data in XML | ![]() | 16 KB | Display | |
Data in CIF | ![]() | 24.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 2bodC ![]() 2bofC ![]() 2bogC ![]() 1tmlS S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 30360.695 Da / Num. of mol.: 1 / Fragment: CATALYTIC DOMAIN, RESIDUES 32-317 / Mutation: YES Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
Compound details | ENDOHYDROL |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 1.9 Å3/Da / Density % sol: 35.6 % |
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Crystal grow | Details: 20-30% POLYETHYLENGLYCOL 4000 AND 0.15-0.34 M SODIUM MALONATE, PH 4.0 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: Dec 14, 2002 / Details: SAGITALLY FOCUSING GE(220) AND A MULTILAYER |
Radiation | Monochromator: DIAMOND (111), GE(220) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.934 Å / Relative weight: 1 |
Reflection | Resolution: 1.15→38.1 Å / Num. obs: 82912 / % possible obs: 100 % / Observed criterion σ(I): 6 / Redundancy: 6.9 % / Rmerge(I) obs: 0.06 / Rsym value: 0.057 / Net I/σ(I): 18.7 |
Reflection shell | Resolution: 1.15→1.16 Å / Redundancy: 6.3 % / Rmerge(I) obs: 0.35 / Mean I/σ(I) obs: 4.9 / Rsym value: 0.318 / % possible all: 100 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1TML Resolution: 1.15→38.07 Å / Cor.coef. Fo:Fc: 0.97 / Cor.coef. Fo:Fc free: 0.955 / SU B: 0.464 / SU ML: 0.023 / Cross valid method: THROUGHOUT / ESU R: 0.041 / ESU R Free: 0.041 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS.
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 11.46 Å2
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Refinement step | Cycle: LAST / Resolution: 1.15→38.07 Å
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Refine LS restraints |
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