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Yorodumi- PDB-6bxs: Crystal structure of Toxoplasma gondii Mitochondrial Association ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 6bxs | ||||||
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Title | Crystal structure of Toxoplasma gondii Mitochondrial Association Factor 1 A (MAF1A) | ||||||
Components | mitochondrial association factor 1 | ||||||
Keywords | MEMBRANE PROTEIN / macro domain / ADPribose / neofunctionalize | ||||||
Function / homology | symbiont-containing vacuole membrane / membrane / Mitochondrial association factor 1 form a1 Function and homology information | ||||||
Biological species | Toxoplasma gondii (eukaryote) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / molecular replacement / Resolution: 2.1 Å | ||||||
Authors | Ramaswamy, R. / Boulanger, M.J. | ||||||
Funding support | Canada, 1items
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Citation | Journal: Mol. Microbiol. / Year: 2018 Title: A Toxoplasma gondii locus required for the direct manipulation of host mitochondria has maintained multiple ancestral functions. Authors: Blank, M.L. / Parker, M.L. / Ramaswamy, R. / Powell, C.J. / English, E.D. / Adomako-Ankomah, Y. / Pernas, L.F. / Workman, S.D. / Boothroyd, J.C. / Boulanger, M.J. / Boyle, J.P. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 6bxs.cif.gz | 169.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb6bxs.ent.gz | 133.8 KB | Display | PDB format |
PDBx/mmJSON format | 6bxs.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/bx/6bxs ftp://data.pdbj.org/pub/pdb/validation_reports/bx/6bxs | HTTPS FTP |
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-Related structure data
Related structure data | 6bxrSC 6bxtC 6bxwC S: Starting model for refinement C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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3 |
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Unit cell |
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-Components
#1: Protein | Mass: 30857.760 Da / Num. of mol.: 3 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Toxoplasma gondii (eukaryote) / Gene: MAF1 / Plasmid: pET28A / Production host: Escherichia coli (E. coli) / Strain (production host): TGGT1_279100 / Variant (production host): ATCC 50853 / GT1 / References: UniProt: A0A193AUK9 #2: Chemical | ChemComp-SO4 / #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.43 Å3/Da / Density % sol: 49.36 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 7 Details: 0.9 M ammonium sulfate, 0.1M Hepes pH 7.0, 0.5% PEG8000, 3% 2- methyl- 2,4- pentanediol |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: CLSI / Beamline: 08ID-1 / Wavelength: 0.9794 Å |
Detector | Type: RAYONIX MX300HE / Detector: CCD / Date: May 27, 2014 Details: Vertical Focusing Mirror: ultra-low expansion (ULE) titanium siliicate flat mirror with Pt, Uncoated, and Pd strips |
Radiation | Monochromator: ACCEL/BRUKER double crystal monochromator (DCM) Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9794 Å / Relative weight: 1 |
Reflection | Resolution: 2.1→48.86 Å / Num. obs: 51862 / % possible obs: 98.5 % / Redundancy: 3.8 % / Rmerge(I) obs: 0.08 / Net I/σ(I): 11.5 |
Reflection shell | Resolution: 2.1→2.16 Å / Redundancy: 3.6 % / Rmerge(I) obs: 0.444 / Mean I/σ(I) obs: 3 / % possible all: 92.4 |
-Phasing
Phasing | Method: molecular replacement |
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-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: 6BXR Resolution: 2.1→48.86 Å / SU ML: 0.24 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 25.92
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso max: 112.37 Å2 / Biso mean: 33.6138 Å2 / Biso min: 15.37 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: final / Resolution: 2.1→48.86 Å
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Refine LS restraints |
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LS refinement shell | Refine-ID: X-RAY DIFFRACTION / Total num. of bins used: 19
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