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- PDB-2abk: REFINEMENT OF THE NATIVE STRUCTURE OF ENDONUCLEASE III TO A RESOL... -
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Open data
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Basic information
Entry | Database: PDB / ID: 2abk | |||||||||
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Title | REFINEMENT OF THE NATIVE STRUCTURE OF ENDONUCLEASE III TO A RESOLUTION OF 1.85 ANGSTROM | |||||||||
![]() | ENDONUCLEASE III | |||||||||
![]() | ENDONUCLEASE / DNA-REPAIR / DNA GLYCOSYLASE | |||||||||
Function / homology | ![]() oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity / base-excision repair, AP site formation via deaminated base removal / base-excision repair, AP site formation / DNA N-glycosylase activity / uracil DNA N-glycosylase activity / DNA-(apurinic or apyrimidinic site) endonuclease activity / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / cellular response to UV / 4 iron, 4 sulfur cluster binding ...oxidized pyrimidine nucleobase lesion DNA N-glycosylase activity / base-excision repair, AP site formation via deaminated base removal / base-excision repair, AP site formation / DNA N-glycosylase activity / uracil DNA N-glycosylase activity / DNA-(apurinic or apyrimidinic site) endonuclease activity / class I DNA-(apurinic or apyrimidinic site) endonuclease activity / DNA-(apurinic or apyrimidinic site) lyase / cellular response to UV / 4 iron, 4 sulfur cluster binding / DNA binding / metal ion binding Similarity search - Function | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() | |||||||||
![]() | Thayer, M.M. / Tainer, J.A. | |||||||||
![]() | ![]() Title: Novel DNA binding motifs in the DNA repair enzyme endonuclease III crystal structure. Authors: Thayer, M.M. / Ahern, H. / Xing, D. / Cunningham, R.P. / Tainer, J.A. #1: ![]() Title: Crystallization and Crystallographic Characterization of the Iron-Sulfur Containing DNA-Repair Enzyme Endonuclease III from Escherichia Coli Enzyme Endonuclease III Authors: Kuo, C.-F. / Mcree, D.E. / Cunningham, R.P. / Tainer, J.A. #2: ![]() Title: Atomic Structure of the DNA Repair [4Fe-4S] Enzyme Endonuclease III Authors: Kuo, C.-F. / Mcree, D.E. / Fisher, C.L. / Handley, S. / Cunningham, R.P. / Tainer, J.A. | |||||||||
History |
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Remark 650 | HELIX DETERMINATION METHOD: KABSCH AND SANDER AS IMPLEMENTED BY PROCHECK FOLLOWED BY VISUAL ...HELIX DETERMINATION METHOD: KABSCH AND SANDER AS IMPLEMENTED BY PROCHECK FOLLOWED BY VISUAL INSPECTION OF MODEL. TURN KABSCH AND SANDER IN PROCHECK TURN_ID: FG, HAIRPIN OF HELIX HAIRPIN HELIX MOTIF. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 55.4 KB | Display | ![]() |
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PDB format | ![]() | 40.7 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 381.4 KB | Display | ![]() |
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Full document | ![]() | 382.7 KB | Display | |
Data in XML | ![]() | 5.8 KB | Display | |
Data in CIF | ![]() | 8.9 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 23595.398 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() References: UniProt: P0AB83, DNA-(apurinic or apyrimidinic site) lyase |
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#2: Chemical | ChemComp-SF4 / |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.93 Å3/Da / Density % sol: 58.07 % | ||||||||||||||||||||||||
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Crystal grow | *PLUS pH: 7 / Method: unknown | ||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Wavelength: 1.5418 Å |
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Detector | Type: XENTRONICS / Detector: AREA DETECTOR / Date: May 18, 1993 |
Radiation | Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.85→20 Å / Num. obs: 27787 / % possible obs: 99.8 % / Observed criterion σ(I): 0 / Redundancy: 10 % / Rmerge(I) obs: 0.07 |
Reflection | *PLUS Rmerge(I) obs: 0.07 |
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Processing
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Refinement | Resolution: 1.85→10 Å / σ(F): 2
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Displacement parameters | Biso mean: 23.7 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.2 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.85→10 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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