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Yorodumi- PDB-1zbn: Solution structure of BIV TAR hairpin complexed to JDV Tat argini... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1zbn | ||||||
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Title | Solution structure of BIV TAR hairpin complexed to JDV Tat arginine-rich motif | ||||||
Components |
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Keywords | RNA binding protein/RNA / RNA-peptide complex / RNA binding protein-RNA COMPLEX | ||||||
Function / homology | Function and homology information positive regulation of viral transcription / host cell nucleolus / RNA-binding transcription regulator activity / viral process / RNA binding Similarity search - Function | ||||||
Method | SOLUTION NMR / distance geometry, restrained molecular dynamics | ||||||
Authors | Calabro, V. / Daugherty, M.D. / Frankel, A.D. | ||||||
Citation | Journal: Proc.Natl.Acad.Sci.Usa / Year: 2005 Title: A single intermolecular contact mediates intramolecular stabilization of both RNA and protein. Authors: Calabro, V. / Daugherty, M.D. / Frankel, A.D. #1: Journal: Mol.Cell / Year: 2000 Title: An RNA-binding chameleon Authors: Smith, C.A. / Calabro, V. / Frankel, A.D. #2: Journal: Science / Year: 1995 Title: Solution structure of a bovine immunodeficiency virus Tat-TAR peptide-RNA complex Authors: Puglisi, J.D. / Chen, L. / Blanchard, S. / Frankel, A.D. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1zbn.cif.gz | 243.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1zbn.ent.gz | 201.1 KB | Display | PDB format |
PDBx/mmJSON format | 1zbn.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1zbn_validation.pdf.gz | 364.8 KB | Display | wwPDB validaton report |
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Full document | 1zbn_full_validation.pdf.gz | 504.3 KB | Display | |
Data in XML | 1zbn_validation.xml.gz | 19.6 KB | Display | |
Data in CIF | 1zbn_validation.cif.gz | 30.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/zb/1zbn ftp://data.pdbj.org/pub/pdb/validation_reports/zb/1zbn | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: RNA chain | Mass: 8923.310 Da / Num. of mol.: 1 / Mutation: A4G, U31C / Source method: obtained synthetically Details: This sequence occurs naturally in Bovine immunodeficiency virus (BIV). |
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#2: Protein/peptide | Mass: 2064.493 Da / Num. of mol.: 1 / Fragment: Arginine-rich domain / Source method: obtained synthetically Details: This sequence occurs naturally in Jembrana disease virus (JDV). References: UniProt: Q82854*PLUS |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: This structure was determined using standard 2D homonuclear techniques. |
-Sample preparation
Details |
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Sample conditions |
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-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian UNITY / Manufacturer: Varian / Model: UNITY / Field strength: 600 MHz |
-Processing
NMR software |
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Refinement | Method: distance geometry, restrained molecular dynamics / Software ordinal: 1 Details: Structures were calculated beginning with 1,000 random structures and incrementally adding distance constraints in four iterations. The final 30 structures with the lowest number of ...Details: Structures were calculated beginning with 1,000 random structures and incrementally adding distance constraints in four iterations. The final 30 structures with the lowest number of violations were subjected to RMD. The structures are based on a total of 700 distance constraints, 61 dihedral angle restraints. | ||||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 30 / Conformers submitted total number: 10 |