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Open data
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Basic information
Entry | Database: PDB / ID: 1zah | ||||||
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Title | Fructose-1,6-bisphosphate aldolase from rabbit muscle | ||||||
![]() | Fructose-bisphosphate aldolase A | ||||||
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Function / homology | ![]() negative regulation of Arp2/3 complex-mediated actin nucleation / ![]() ![]() ![]() Similarity search - Function | ||||||
Biological species | ![]() ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | St-Jean, M. / Lafrance-Vanasse, J. / Liotard, B. / Sygusch, J. | ||||||
![]() | ![]() Title: High Resolution Reaction Intermediates of Rabbit Muscle Fructose-1,6-bisphosphate Aldolase: substrate cleavage and induced fit. Authors: St-Jean, M. / Lafrance-Vanasse, J. / Liotard, B. / Sygusch, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 328.8 KB | Display | ![]() |
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PDB format | ![]() | 273.8 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 319.3 KB | Display | ![]() |
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Full document | ![]() | 342.1 KB | Display | |
Data in XML | ![]() | 81.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1zaiC ![]() 1zajC ![]() 1zalC ![]() 1adoS C: citing same article ( S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Details | The biological assembly is the homotetramer found in the asymmetric unit |
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Components
#1: Protein | Mass: 39263.672 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | ![]() |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.28 Å3/Da / Density % sol: 46.1 % |
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Crystal grow![]() | Temperature: 296 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: sodium HEPES, PEG 4000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Apr 1, 2001 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength![]() |
Reflection | Resolution: 1.8→99 Å / Num. all: 131029 / Num. obs: 127047 / % possible obs: 97 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.049 / Χ2: 0.972 |
Reflection shell | Resolution: 1.8→1.86 Å / % possible obs: 87.1 % / Rmerge(I) obs: 0.388 / Num. measured obs: 11347 / Χ2: 1.029 / % possible all: 87.1 |
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Processing
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Refinement | Method to determine structure![]() ![]() Starting model: PDB ENTRY 1ADO Resolution: 1.8→50 Å / Cross valid method: THROUGHOUT / σ(F): 0 / σ(I): 1 / Stereochemistry target values: Engh & Huber
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Solvent computation | Bsol: 71.666 Å2 | |||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 27.69 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.8→50 Å
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Refine LS restraints |
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Xplor file |
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