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Yorodumi- PDB-1z7w: Crystal Structure of O-Acetylserine Sulfhydrylase from Arabidopsi... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1z7w | ||||||
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Title | Crystal Structure of O-Acetylserine Sulfhydrylase from Arabidopsis thaliana | ||||||
Components | Cysteine synthase | ||||||
Keywords | TRANSFERASE | ||||||
Function / homology | Function and homology information double fertilization forming a zygote and endosperm / pollen tube growth / cysteine synthase / cysteine synthase activity / cysteine biosynthetic process / apoplast / plant-type vacuole / cysteine biosynthetic process from serine / chloroplast stroma / response to cadmium ion ...double fertilization forming a zygote and endosperm / pollen tube growth / cysteine synthase / cysteine synthase activity / cysteine biosynthetic process / apoplast / plant-type vacuole / cysteine biosynthetic process from serine / chloroplast stroma / response to cadmium ion / chloroplast / peroxisome / mRNA binding / nucleus / plasma membrane / cytosol Similarity search - Function | ||||||
Biological species | Arabidopsis thaliana (thale cress) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.2 Å | ||||||
Authors | Bonner, E.R. / Cahoon, R.E. / Knapke, S.M. / Jez, J.M. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2005 Title: Molecular Basis of Cysteine Biosynthesis in Plants: STRUCTURAL AND FUNCTIONAL ANALYSIS OF O-ACETYLSERINE SULFHYDRYLASE FROM ARABIDOPSIS THALIANA. Authors: Bonner, E.R. / Cahoon, R.E. / Knapke, S.M. / Jez, J.M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1z7w.cif.gz | 76.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1z7w.ent.gz | 56.4 KB | Display | PDB format |
PDBx/mmJSON format | 1z7w.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/z7/1z7w ftp://data.pdbj.org/pub/pdb/validation_reports/z7/1z7w | HTTPS FTP |
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-Related structure data
Related structure data | 1z7yC 1oasS C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 33840.027 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Arabidopsis thaliana (thale cress) / Gene: OASA1, OAS1 / Plasmid: pET28a-AtOASS / Species (production host): Escherichia coli / Production host: Escherichia coli BL21(DE3) (bacteria) / Strain (production host): BL21(DE3) / References: UniProt: P47998, cysteine synthase |
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#2: Chemical | ChemComp-SO4 / |
#3: Chemical | ChemComp-PLP / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.03 Å3/Da / Density % sol: 39.48 % |
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop / pH: 8 Details: ammonium sulfate, Tris, pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: ENRAF-NONIUS FR591 / Wavelength: 1.5418 |
Detector | Type: BRUKER SMART 6000 / Detector: CCD / Date: Dec 30, 2003 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→75 Å / Num. all: 29108 / Num. obs: 26988 / % possible obs: 92.7 % / Observed criterion σ(I): 0 |
Reflection shell | Resolution: 2.2→2.3 Å / % possible all: 88.1 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB Entry 1OAS Resolution: 2.2→15 Å / σ(F): 2 / Stereochemistry target values: Engh & Huber
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Refinement step | Cycle: LAST / Resolution: 2.2→15 Å
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Refine LS restraints |
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