+Open data
-Basic information
Entry | Database: PDB / ID: 1oas | ||||||
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Title | O-ACETYLSERINE SULFHYDRYLASE FROM SALMONELLA TYPHIMURIUM | ||||||
Components | O-ACETYLSERINE SULFHYDRYLASE | ||||||
Keywords | LYASE / CYSTEIN BIOSYNTHESIS / BETA REPLACEMENT ENZYME / PLP DEPENDENT ENZYME / HOMODIMER | ||||||
Function / homology | Function and homology information cysteine synthase / L-cysteine desulfhydrase activity / cysteine synthase activity / cysteine biosynthetic process from serine / cytoplasm Similarity search - Function | ||||||
Biological species | Salmonella typhimurium (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MIR / Resolution: 2.2 Å | ||||||
Authors | Burkhard, P. / Rao, G.S.J. / Hohenester, E. / Schnackerz, K.D. / Cook, P.F. / Jansonius, J.N. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 1998 Title: Three-dimensional structure of O-acetylserine sulfhydrylase from Salmonella typhimurium. Authors: Burkhard, P. / Rao, G.S. / Hohenester, E. / Schnackerz, K.D. / Cook, P.F. / Jansonius, J.N. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1oas.cif.gz | 137 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1oas.ent.gz | 108.3 KB | Display | PDB format |
PDBx/mmJSON format | 1oas.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/oa/1oas ftp://data.pdbj.org/pub/pdb/validation_reports/oa/1oas | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (0.383461, -0.575913, -0.721999), Vector: |
-Components
#1: Protein | Mass: 34470.422 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Salmonella typhimurium (bacteria) / Production host: Salmonella typhimurium (bacteria) / References: UniProt: P0A1E3, EC: 4.2.99.8 #2: Chemical | #3: Water | ChemComp-HOH / | Nonpolymer details | SCHIFF-BASE LINKAGE TO K41 A SCHIFF-BASE LINKAGE TO K41 B | Sequence details | the sequence differences reported in SEQADV are probably sequencing mistakes. | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.83 Å3/Da / Density % sol: 56.47 % | ||||||||||||||||||||||||||||||
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Crystal grow | pH: 7 / Details: pH 7.00 | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS pH: 8 / Method: vapor diffusion, hanging drop | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 287 K |
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Diffraction source | Source: SYNCHROTRON / Site: ESRF / Beamline: BM1A / Wavelength: 1.02 |
Detector | Type: MARRESEARCH / Detector: AREA DETECTOR / Date: Apr 1, 1997 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.02 Å / Relative weight: 1 |
Reflection | Resolution: 2.15→50 Å / Num. obs: 43785 / % possible obs: 96.8 % / Observed criterion σ(I): 0 / Redundancy: 3 % / Biso Wilson estimate: 18.6 Å2 / Rmerge(I) obs: 0.064 |
Reflection | *PLUS Num. measured all: 155500 |
-Processing
Software |
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Refinement | Method to determine structure: MIR / Resolution: 2.2→20 Å / Rfactor Rfree error: 0.006 / Data cutoff high rms absF: 2316675.99 / Isotropic thermal model: RESTRAINED / Cross valid method: A POSTERIORI / σ(F): 0
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 80.69 Å2 / ksol: 0.337 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 36.3 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.2→20 Å
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Refine LS restraints |
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Refine LS restraints NCS | NCS model details: CONSTR | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
LS refinement shell | Resolution: 2.2→2.34 Å / Rfactor Rfree error: 0.02 / Total num. of bins used: 6
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Xplor file |
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Software | *PLUS Name: CNS / Version: 0.5 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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