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- PDB-7djq: Crystal Structure of O-acetyl L-serine sulfhydrylase from Haemoph... -
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Open data
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Basic information
Entry | Database: PDB / ID: 7djq | ||||||
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Title | Crystal Structure of O-acetyl L-serine sulfhydrylase from Haemophilus influenzae in complex with C-Terminal peptide of ribosomal S4 Domain protein from Lactobacillus salivarius. | ||||||
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![]() | TRANSFERASE / Complex / Enzyme / inhibitor | ||||||
Function / homology | ![]() cysteine synthase / cystathionine beta-synthase activity / cysteine synthase activity / L-cysteine desulfhydrase activity / cysteine biosynthetic process from serine / RNA binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Saini, N. / Rahisuddin, R. / Kumaran, S. | ||||||
![]() | ![]() Title: Moonlighting Biochemistry of Cysteine Synthase: A Species-specific Global Regulator. Authors: Singh, R.P. / Saini, N. / Sharma, G. / Rahisuddin, R. / Patel, M. / Kaushik, A. / Kumaran, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 220.6 KB | Display | ![]() |
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PDB format | ![]() | 173.9 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.6 MB | Display | ![]() |
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Full document | ![]() | 1.6 MB | Display | |
Data in XML | ![]() | 23.2 KB | Display | |
Data in CIF | ![]() | 32.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 4ho1S S: Starting model for refinement |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein/peptide | Mass: 1188.221 Da / Num. of mol.: 1 / Source method: obtained synthetically / Source: (synth.) ![]() | ||||||
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#2: Protein | Mass: 37244.453 Da / Num. of mol.: 2 / Mutation: D67E/A68P Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() Gene: cysK, HI_1103 Production host: ![]() ![]() References: UniProt: P45040, cysteine synthase #3: Chemical | #4: Water | ChemComp-HOH / | Has ligand of interest | Y | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.33 Å3/Da / Density % sol: 47.1 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, sitting drop / pH: 7.4 / Details: 100mM HEPES buffer pH 7.4, 1.4M Sodium Citrate |
-Data collection
Diffraction | Mean temperature: 100 K / Serial crystal experiment: N |
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Diffraction source | Source: ![]() |
Detector | Type: MAR scanner 345 mm plate / Detector: IMAGE PLATE / Date: Mar 19, 2020 |
Radiation | Monochromator: M / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→40.86 Å / Num. obs: 28307 / % possible obs: 97.66 % / Redundancy: 15.2 % / Biso Wilson estimate: 19.86 Å2 / CC1/2: 0.855 / CC star: 0.96 / Rmerge(I) obs: 0.063 / Net I/σ(I): 17.1 |
Reflection shell | Resolution: 2.3→2.382 Å / Redundancy: 9 % / Rmerge(I) obs: 0.15 / Num. unique obs: 2705 / CC1/2: 0.826 / CC star: 0.951 / % possible all: 94.98 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: 4HO1 Resolution: 2.3→40.86 Å / SU ML: 0.2184 / Cross valid method: FREE R-VALUE / σ(F): 1.35 / Phase error: 23.1909 / Stereochemistry target values: CDL v1.2
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Solvent computation | Shrinkage radii: 0.9 Å / VDW probe radii: 1.11 Å / Solvent model: FLAT BULK SOLVENT MODEL | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 22.76 Å2 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.3→40.86 Å
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Refine LS restraints |
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LS refinement shell |
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