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- PDB-1yzf: Crystal structure of the lipase/acylhydrolase from Enterococcus f... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1yzf | ||||||
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Title | Crystal structure of the lipase/acylhydrolase from Enterococcus faecalis | ||||||
![]() | lipase/acylhydrolase | ||||||
![]() | HYDROLASE / Enterococcus faecalis / lipase/acylhydrolase / Structural Genomics / PSI / Protein Structure Initiative / Midwest Center for Structural Genomics / MCSG | ||||||
Function / homology | SGNH hydrolase / SGNH hydrolase-type esterase domain / GDSL-like Lipase/Acylhydrolase family / SGNH hydrolase superfamily / hydrolase activity / Rossmann fold / 3-Layer(aba) Sandwich / Alpha Beta / Lipase/acylhydrolase![]() | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Zhang, R. / Hatzos, C. / Clancy, S. / Collart, F. / Joachimiak, A. / Midwest Center for Structural Genomics (MCSG) | ||||||
![]() | ![]() Title: Crystal structure of the lipase/acylhydrolase from Enterococcus faecalis Authors: Zhang, R. / Hatzos, C. / Clancy, S. / Collart, F. / Joachimiak, A. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 54.8 KB | Display | ![]() |
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PDB format | ![]() | 39.4 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 412 KB | Display | ![]() |
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Full document | ![]() | 413.5 KB | Display | |
Data in XML | ![]() | 11.9 KB | Display | |
Data in CIF | ![]() | 17.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Details | This protein existed as monomer, Molecule A represents the monomer in the assymtric unit. |
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Components
#1: Protein | Mass: 21495.756 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.013 Å3/Da / Density % sol: 36.55 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, sitting drop / pH: 6 Details: 30% PEG6000, 0.1M MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Feb 18, 2005 / Details: mirrors |
Radiation | Monochromator: Si 111 channel / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.9795 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→50 Å / Num. obs: 15100 / % possible obs: 97.1 % / Observed criterion σ(F): 2 / Observed criterion σ(I): 2 / Redundancy: 6.3 % / Biso Wilson estimate: 25.5 Å2 / Rmerge(I) obs: 0.113 / Net I/σ(I): 17.09 |
Reflection shell | Resolution: 1.8→1.86 Å / Redundancy: 2.3 % / Rmerge(I) obs: 0.337 / Mean I/σ(I) obs: 1.6 / Num. unique all: 3230 / % possible all: 74.7 |
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Processing
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Refinement | Method to determine structure: ![]() Stereochemistry target values: MAXIMUM LIKELIHOOD WITH PHASES Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 25.495 Å2
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Refine analyze | Luzzati coordinate error obs: 0.176 Å / Luzzati d res low obs: 5 Å / Luzzati sigma a obs: 0.163 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.9→49.33 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.896→1.945 Å / Total num. of bins used: 20
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