Entry Database : PDB / ID : 1y27 Structure visualization Downloads & linksTitle G-riboswitch-guanine complex ComponentsBacillus subtilis xpt Details Keywords RNA / G-riboswitch guanine recognitionFunction / homology GUANINE / RNA / RNA (> 10) Function and homology informationBiological species Bacillus subtilis (bacteria)Method X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution : 2.4 Å DetailsAuthors Serganov, A. / Yuan, Y.R. / Patel, D.J. CitationJournal : Chem.Biol. / Year : 2004Title : Structural Basis for Discriminative Regulation of Gene Expression by Adenine- and Guanine-Sensing mRNAsAuthors : Serganov, A. / Yuan, Y.R. / Pikovskaya, O. / Polonskaia, A. / Malinina, L. / Phan, A.T. / Hobartner, C. / Micura, R. / Breaker, R.R. / Patel, D.J. History Deposition Nov 20, 2004 Deposition site : RCSB / Processing site : RCSBRevision 1.0 Dec 28, 2004 Provider : repository / Type : Initial releaseRevision 1.1 Apr 30, 2008 Group : Version format complianceRevision 1.2 Jul 13, 2011 Group : Advisory / Version format complianceRevision 1.3 Oct 11, 2017 Group : Refinement description / Category : software / Item : _software.classification / _software.nameRevision 2.0 Feb 27, 2019 Group : Advisory / Atomic model ... Advisory / Atomic model / Data collection / Database references / Derived calculations / Non-polymer description / Polymer sequence / Refinement description / Source and taxonomy / Structure summary Category : atom_site / chem_comp ... atom_site / chem_comp / entity / entity_name_com / entity_poly / entity_poly_seq / entity_src_gen / ndb_struct_na_base_pair / ndb_struct_na_base_pair_step / pdbx_entity_nonpoly / pdbx_nonpoly_scheme / pdbx_poly_seq_scheme / pdbx_refine_tls_group / pdbx_struct_assembly / pdbx_struct_assembly_gen / pdbx_unobs_or_zero_occ_atoms / pdbx_validate_close_contact / pdbx_validate_planes / pdbx_validate_rmsd_angle / pdbx_validate_rmsd_bond / refine / refine_hist / refine_ls_restr / refine_ls_shell / reflns / reflns_shell / struct_asym / struct_conn / struct_ref / struct_site / struct_site_gen Item : _atom_site.B_iso_or_equiv / _atom_site.Cartn_x ... _atom_site.B_iso_or_equiv / _atom_site.Cartn_x / _atom_site.Cartn_y / _atom_site.Cartn_z / _atom_site.auth_atom_id / _atom_site.auth_comp_id / _atom_site.auth_seq_id / _atom_site.group_PDB / _atom_site.label_asym_id / _atom_site.label_atom_id / _atom_site.label_comp_id / _atom_site.label_entity_id / _atom_site.label_seq_id / _atom_site.type_symbol / _chem_comp.formula / _chem_comp.formula_weight / _chem_comp.id / _chem_comp.mon_nstd_flag / _chem_comp.name / _chem_comp.type / _entity_poly.pdbx_seq_one_letter_code / _entity_poly_seq.mon_id / _entity_src_gen.pdbx_beg_seq_num / _entity_src_gen.pdbx_end_seq_num / _entity_src_gen.pdbx_seq_type / _ndb_struct_na_base_pair.buckle / _ndb_struct_na_base_pair.hbond_type_12 / _ndb_struct_na_base_pair.hbond_type_28 / _ndb_struct_na_base_pair.i_auth_seq_id / _ndb_struct_na_base_pair.i_label_comp_id / _ndb_struct_na_base_pair.i_label_seq_id / _ndb_struct_na_base_pair.j_auth_seq_id / _ndb_struct_na_base_pair.j_label_comp_id / _ndb_struct_na_base_pair.j_label_seq_id / _ndb_struct_na_base_pair.opening / _ndb_struct_na_base_pair.pair_name / _ndb_struct_na_base_pair.propeller / _ndb_struct_na_base_pair.shear / _ndb_struct_na_base_pair.stagger / _ndb_struct_na_base_pair.stretch / _pdbx_poly_seq_scheme.auth_mon_id / _pdbx_poly_seq_scheme.mon_id / _pdbx_poly_seq_scheme.pdb_mon_id / _pdbx_refine_tls_group.beg_label_asym_id / _pdbx_refine_tls_group.beg_label_seq_id / _pdbx_refine_tls_group.end_label_asym_id / _pdbx_refine_tls_group.end_label_seq_id / _pdbx_struct_assembly.details / _pdbx_struct_assembly.method_details / _pdbx_struct_assembly_gen.asym_id_list / _refine.B_iso_mean / _refine.details / _refine.ls_R_factor_R_free / _refine.ls_R_factor_R_work / _refine.ls_R_factor_obs / _refine.ls_percent_reflns_obs / _refine.pdbx_ls_sigma_F / _refine_hist.pdbx_number_atoms_ligand / _refine_hist.pdbx_number_atoms_nucleic_acid / _refine_ls_shell.d_res_low / _reflns.d_resolution_low / _reflns.number_all / _reflns.observed_criterion_sigma_F / _reflns.percent_possible_obs / _reflns_shell.number_unique_all / _reflns_shell.percent_possible_all / _struct_ref.pdbx_align_begin Revision 2.1 Aug 23, 2023 Group : Data collection / Database references / Refinement descriptionCategory : chem_comp_atom / chem_comp_bond ... chem_comp_atom / chem_comp_bond / database_2 / pdbx_initial_refinement_model Item : _database_2.pdbx_DOI / _database_2.pdbx_database_accession
Show all Show less Remark 999 SEQUENCE THE G-RIBOSWITCH SEQUENCE WAS DERIVED FROM THE SEQUENCE LOCATED UPSTREAM OF THE XPT GENE ... SEQUENCE THE G-RIBOSWITCH SEQUENCE WAS DERIVED FROM THE SEQUENCE LOCATED UPSTREAM OF THE XPT GENE (XPT-PBUX OPERON) ENCODING FOR THE XANTHINE PHOPHORIBOSYLTRANSFERASE FROM BACILLUS SUBTILIS. THE STRUCTURE IN THIS ENTRY IS A COMPLEX BETWEEN GUANINE AND A DOMAIN (APTAMER DOMAIN) OF THE RIBOSWITCH, WHICH SHUTS DOWN TRANSCRIPTION OF THE XPT GENE IN RESPONSE TO THE PRESENCE OF PURINES IN THE CELL. THIS SEQUENCE CAN BE FOUND NATURALLY AND THE DATABASE MATCH IS FOUND IN BACILLUS SUBTILIS GENEBANK ENTRY 633168 X83878 (RESIDUES 185-252).