+Open data
-Basic information
Entry | Database: PDB / ID: 1xwo | ||||||
---|---|---|---|---|---|---|---|
Title | crystal structrue of goose delta crystallin | ||||||
Components | Delta crystallin | ||||||
Keywords | LYASE / Alpha helix bundle / double dimer | ||||||
Function / homology | Function and homology information argininosuccinate lyase / argininosuccinate lyase activity / arginine biosynthetic process via ornithine / structural constituent of eye lens / arginine biosynthetic process Similarity search - Function | ||||||
Biological species | Anser anser anser (western graylag goose) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.8 Å | ||||||
Authors | Lee, H.J. / Lai, Y.H. / Wu, S.Y. / Chang, G.G. | ||||||
Citation | Journal: Biochem.J. / Year: 2005 Title: The effect of N-terminal truncation on double-dimer assembly of goose delta-crystallin Authors: Lee, H.J. / Lai, Y.H. / Wu, S.Y. / Chen, Y.H. | ||||||
History |
|
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
---|
-Downloads & links
-Download
PDBx/mmCIF format | 1xwo.cif.gz | 342.6 KB | Display | PDBx/mmCIF format |
---|---|---|---|---|
PDB format | pdb1xwo.ent.gz | 282.4 KB | Display | PDB format |
PDBx/mmJSON format | 1xwo.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/xw/1xwo ftp://data.pdbj.org/pub/pdb/validation_reports/xw/1xwo | HTTPS FTP |
---|
-Related structure data
Similar structure data |
---|
-Links
-Assembly
Deposited unit |
| ||||||||
---|---|---|---|---|---|---|---|---|---|
1 |
| ||||||||
Unit cell |
|
-Components
#1: Protein | Mass: 51300.863 Da / Num. of mol.: 4 / Source method: isolated from a natural source / Source: (natural) Anser anser anser (western graylag goose) / Species: Anser anser / Strain: anser / Tissue: Eye lensLens (anatomy) / References: UniProt: P33110, argininosuccinate lyase #2: Water | ChemComp-HOH / | |
---|
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
---|
-Sample preparation
Crystal | Density Matthews: 2.4 Å3/Da / Density % sol: 48.8 % |
---|---|
Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.5 Details: PEG 2000, magnesium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
---|---|
Diffraction source | Source: ROTATING ANODE / Type: RIGAKU / Wavelength: 1.542 Å |
Detector | Type: RIGAKU RAXIS IV / Detector: IMAGE PLATE / Date: Oct 10, 2003 |
Radiation | Monochromator: GRAPHITE / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.542 Å / Relative weight: 1 |
Reflection | Resolution: 2.8→20 Å / Num. all: 45901 / Num. obs: 45763 / % possible obs: 99.7 % / Observed criterion σ(F): 1.6 / Observed criterion σ(I): 1.6 / Redundancy: 16.4 % / Rmerge(I) obs: 0.145 / Rsym value: 0.145 |
Reflection shell | Resolution: 2.8→2.85 Å / % possible all: 99.1 |
-Processing
Software |
| ||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.8→20 Å / σ(F): 1.6 / Stereochemistry target values: Engh & Huber
| ||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 2.8→20 Å
| ||||||||||||||||||||
Refine LS restraints |
| ||||||||||||||||||||
LS refinement shell | Resolution: 2.8→2.85 Å
|