+Open data
-Basic information
Entry | Database: PDB / ID: 1xps | ||||||
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Title | BOVINE RIBONUCLEASE A (PHOSPHATE-FREE) (93 % HUMIDITY) | ||||||
Components | RIBONUCLEASE A | ||||||
Keywords | HYDROLASE / HYDROLASE (PHOSPHORIC DIESTER) | ||||||
Function / homology | Function and homology information pancreatic ribonuclease / ribonuclease A activity / RNA nuclease activity / nucleic acid binding / lyase activity / defense response to Gram-positive bacterium / extracellular region Similarity search - Function | ||||||
Biological species | Bos taurus (cattle) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.8 Å | ||||||
Authors | Sadasivan, C. / Nagendra, H.G. / Vijayan, M. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 1998 Title: Plasticity, hydration and accessibility in ribonuclease A. The structure of a new crystal form and its low-humidity variant. Authors: Sadasivan, C. / Nagendra, H.G. / Vijayan, M. #1: Journal: Acta Crystallogr.,Sect.D / Year: 1995 Title: Water-Dependent Domain Motion and Flexibility in Ribonuclease A and the Invariant Features in its Hydration Shell. An X-Ray Study of Two Low-Humidity Crystal Forms of the Enzyme Authors: Kishan, K.V.R. / Chandra, N.R. / Sudarsanakumar, C. / Suguna, K. / Vijayan, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1xps.cif.gz | 63.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1xps.ent.gz | 47.3 KB | Display | PDB format |
PDBx/mmJSON format | 1xps.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1xps_validation.pdf.gz | 414.9 KB | Display | wwPDB validaton report |
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Full document | 1xps_full_validation.pdf.gz | 415.6 KB | Display | |
Data in XML | 1xps_validation.xml.gz | 13.5 KB | Display | |
Data in CIF | 1xps_validation.cif.gz | 19.5 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/xp/1xps ftp://data.pdbj.org/pub/pdb/validation_reports/xp/1xps | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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Unit cell |
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-Components
#1: Protein | Mass: 13708.326 Da / Num. of mol.: 2 / Source method: isolated from a natural source / Details: PHOSPHATE-FREE / Source: (natural) Bos taurus (cattle) / Organ: PANCREAS / References: UniProt: P61823, EC: 3.1.27.5 #2: Water | ChemComp-HOH / | Compound details | THE STRUCTURE OF LOW HUMIDITY (93% RELATIVE HUMIDITY) FORM A NEW CRYSTAL FORM OF RIBONUCLEASE A AND ...THE STRUCTURE OF LOW HUMIDITY (93% RELATIVE HUMIDITY) FORM A NEW CRYSTAL FORM OF RIBONUCLEA | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 1.94 Å3/Da / Density % sol: 36.53 % | |||||||||||||||
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Crystal | *PLUS Density % sol: 36.6 % | |||||||||||||||
Crystal grow | *PLUS pH: 7.6 / Method: unknown | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | Num. obs: 14420 / % possible obs: 74.6 % / Observed criterion σ(I): 0 / Rmerge(I) obs: 0.0384 |
Reflection | *PLUS Highest resolution: 1.8 Å / Num. measured all: 43080 |
Reflection shell | *PLUS Highest resolution: 1.8 Å / Lowest resolution: 1.9 Å / % possible obs: 51 % |
-Processing
Software |
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Refinement | Resolution: 1.8→10 Å / σ(F): 2
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Displacement parameters | Biso mean: 17.6 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine analyze | Luzzati coordinate error obs: 0.18 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→10 Å
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Refine LS restraints |
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Software | *PLUS Version: 3.1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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