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Yorodumi- PDB-1rha: WATER DEPENDENT DOMAIN MOTION AND FLEXIBILITY IN RIBONUCLEASE A A... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1rha | ||||||
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Title | WATER DEPENDENT DOMAIN MOTION AND FLEXIBILITY IN RIBONUCLEASE A AND THE INVARIANT FEATURES IN ITS HYDRATION SHELL. AN X-RAY STUDY OF TWO LOW HUMIDITY CRYSTAL FORMS OF THE ENZYME | ||||||
Components | RIBONUCLEASE A | ||||||
Keywords | HYDROLASE (NUCLEIC ACID / RNA) | ||||||
Function / homology | Function and homology information pancreatic ribonuclease / ribonuclease A activity / RNA nuclease activity / nucleic acid binding / lyase activity / defense response to Gram-positive bacterium / extracellular region Similarity search - Function | ||||||
Biological species | Bos taurus (cattle) | ||||||
Method | X-RAY DIFFRACTION / Resolution: 1.8 Å | ||||||
Authors | Radha Kishan, K.V. / Chandra, N.R. / Sudarsanakumar, C. / Suguna, K. / Vijayan, M. | ||||||
Citation | Journal: Acta Crystallogr.,Sect.D / Year: 1995 Title: Water-dependent domain motion and flexibility in ribonuclease A and the invariant features in its hydration shell. An X-ray study of two low-humidity crystal forms of the enzyme. Authors: Kishan, R.V. / Chandra, N.R. / Sudarsanakumar, C. / Suguna, K. / Vijayan, M. #1: Journal: Acta Crystallogr.,Sect.D / Year: 1993 Title: Protein Hydration and Water Structure: X-Ray Analysis of a Closely Packed Protein Crystal with Very Low Solvent Content Authors: Madhusudan / Kodandapani, R. / Vijayan, M. #2: Journal: Curr.Sci. / Year: 1991 Title: Rigid and Flexible Regions in Lysozyme and the Invariant Features in its Hydration Shell Authors: Madhusudan / Vijayan, M. #3: Journal: J.Biol.Chem. / Year: 1990 Title: Crystal Structure of Low Humidity Tetragonal Lysozyme at at 2.1 Angstroms Resolution; Variability in Hydration Shell and its Structural Consequences Authors: Kodandapani, R. / Suresh, C.G. / Vijayan, M. #4: Journal: Acta Crystallogr.,Sect.B / Year: 1985 Title: Water-Mediated Transformations in Protein Crystals Authors: Salunke, D.M. / Veerapandian, B. / Kodandapani, R. / Vijayan, M. #5: Journal: Curr.Sci. / Year: 1984 Title: Water-Mediated Structural Transformations in a New Crystal Form of Ribonuclease A and Tetragonal Lysozyme Authors: Salunke, D.M. / Veerapandian, B. / Vijayan, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1rha.cif.gz | 39 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1rha.ent.gz | 26.7 KB | Display | PDB format |
PDBx/mmJSON format | 1rha.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1rha_validation.pdf.gz | 414.6 KB | Display | wwPDB validaton report |
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Full document | 1rha_full_validation.pdf.gz | 419.7 KB | Display | |
Data in XML | 1rha_validation.xml.gz | 9.4 KB | Display | |
Data in CIF | 1rha_validation.cif.gz | 12.9 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rh/1rha ftp://data.pdbj.org/pub/pdb/validation_reports/rh/1rha | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO 93 / 2: CIS PROLINE - PRO 114 |
-Components
#1: Protein | Mass: 13708.326 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Bos taurus (cattle) / Cell line: S2 / Organ: PANCREAS / References: UniProt: P61823, EC: 3.1.27.5 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 1.78 Å3/Da / Density % sol: 30.86 % | |||||||||||||||
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Crystal grow | *PLUS Method: batch method | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Radiation | Scattering type: x-ray |
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Radiation wavelength | Relative weight: 1 |
Reflection | *PLUS Highest resolution: 1.8 Å / Lowest resolution: 10 Å / Num. obs: 8667 / Num. measured all: 17852 / Rmerge(I) obs: 0.027 |
-Processing
Software | Name: PROLSQ / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Refinement | Resolution: 1.8→10 Å / σ(F): 2 /
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Refinement step | Cycle: LAST / Resolution: 1.8→10 Å
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Refine LS restraints |
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