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Open data
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Basic information
Entry | Database: PDB / ID: 1xm3 | ||||||
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Title | Crystal structure of Northeast Structural Genomics Target SR156 | ||||||
![]() | Thiazole biosynthesis protein thiG | ||||||
![]() | BIOSYNTHETIC PROTEIN / Structural Genomics / Protein Structure Initiative / PSI / NESG / Northeast Structural Genomics Consortium | ||||||
Function / homology | ![]() thiazole synthase activity / thiazole synthase / thiamine diphosphate biosynthetic process / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() ![]() | ||||||
![]() | Kuzin, A. / Abashidze, M. / Vorobiev, S. / Forouhar, F. / Acton, T. / Ma, L. / Xiao, R. / Montelione, G. / Tong, L. / Hunt, J. / Northeast Structural Genomics Consortium (NESG) | ||||||
![]() | ![]() Title: Crystal structure of Northeast Structural Genomics Target SR156 Authors: Kuzin, A. / Abashidze, M. / Vorobiev, S. / Forouhar, F. / Acton, T. / Ma, L. / Xiao, R. / Montelione, G. / Tong, L. / Hunt, J. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 198 KB | Display | ![]() |
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PDB format | ![]() | 167.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 461.7 KB | Display | ![]() |
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Full document | ![]() | 483 KB | Display | |
Data in XML | ![]() | 43.8 KB | Display | |
Data in CIF | ![]() | 62.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
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Links
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Assembly
Deposited unit | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 28445.576 Da / Num. of mol.: 4 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.7 Å3/Da / Density % sol: 40 % |
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Crystal grow | Temperature: 291 K / Method: vapor diffusion, hanging drop / pH: 7 Details: PEG 3350, magnesium nitrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97913 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→30 Å / Num. obs: 216070 / Observed criterion σ(I): -3 / Biso Wilson estimate: 17.5 Å2 |
Reflection shell | Resolution: 1.8→1.91 Å / % possible all: 59.4 |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 47.2042 Å2 / ksol: 0.397272 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 23.3 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.8→29.82 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.8→1.91 Å / Rfactor Rfree error: 0.008 / Total num. of bins used: 6
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Xplor file |
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