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Open data
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Basic information
Entry | Database: PDB / ID: 3gjb | ||||||
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Title | CytC3 with Fe(II) and alpha-ketoglutarate | ||||||
![]() | CytC3 | ||||||
![]() | BIOSYNTHETIC PROTEIN / CytC3 / halogenase / beta barrel | ||||||
Function / homology | ![]() | ||||||
Biological species | ![]() | ||||||
Method | ![]() ![]() ![]() ![]() | ||||||
![]() | Wong, C. / Drennan, C.L. | ||||||
![]() | ![]() Title: Structural analysis of an open active site conformation of nonheme iron halogenase CytC3 Authors: Wong, C. / Fujimori, D.G. / Walsh, C.T. / Drennan, C.L. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 125.7 KB | Display | ![]() |
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PDB format | ![]() | 97 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 480.9 KB | Display | ![]() |
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Full document | ![]() | 495.3 KB | Display | |
Data in XML | ![]() | 25.1 KB | Display | |
Data in CIF | ![]() | 34.7 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 | ![]()
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Unit cell |
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Components
-Protein , 1 types, 2 molecules AB
#1: Protein | Mass: 36484.574 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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-Non-polymers , 5 types, 232 molecules ![](data/chem/img/FE2.gif)
![](data/chem/img/AKG.gif)
![](data/chem/img/ACT.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/HOH.gif)
![](data/chem/img/AKG.gif)
![](data/chem/img/ACT.gif)
![](data/chem/img/SO4.gif)
![](data/chem/img/HOH.gif)
#2: Chemical | #3: Chemical | #4: Chemical | ChemComp-ACT / #5: Chemical | #6: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.37 Å3/Da / Density % sol: 63.5 % |
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Crystal grow | Temperature: 298 K / pH: 7 Details: 2.8 M sodium acetate trihydrate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARMOSAIC 325 mm CCD / Detector: CCD / Date: Jul 1, 2006 / Details: INTER-IMAGE DEAD TIME 5S |
Radiation | Monochromator: SIDE SCATTERING BENT CUBE- ROOT I-BEAM SINGLE CRYSTAL Protocol: SINGLE WAVELENGTH / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.2→50 Å / Num. obs: 50583 / % possible obs: 97.6 % / Redundancy: 11 % / Rmerge(I) obs: 0.082 |
Reflection shell | Resolution: 2.2→2.28 Å / Redundancy: 7.1 % / Rmerge(I) obs: 0.48 / % possible all: 81.3 |
-Phasing
Phasing | Method: ![]() |
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Processing
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Refinement | Method to determine structure: ![]()
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Solvent computation | Bsol: 62.81 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 48.04 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.2→44.49 Å
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Refine LS restraints |
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Xplor file |
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