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- PDB-1x93: NMR Structure of Helicobacter pylori HP0222 -

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Basic information

Entry
Database: PDB / ID: 1x93
TitleNMR Structure of Helicobacter pylori HP0222
Componentshypothetical protein HP0222
KeywordsTRANSCRIPTION / HP0222 / JHP0208 / helicobacter / pylori / regulator / Arc / MetJ / Mnt / ParG / CopG / RepA
Function / homology
Function and homology information


regulation of DNA-templated transcription
Similarity search - Function
RHH transcriptional regulator CopG / Transcriptional regulator, RHH-like, CopG / Met repressor-like / Arc Repressor Mutant / Arc-type ribbon-helix-helix / Ribbon-helix-helix / Orthogonal Bundle / Mainly Alpha
Similarity search - Domain/homology
Uncharacterized protein
Similarity search - Component
Biological speciesHelicobacter pylori (bacteria)
MethodSOLUTION NMR
AuthorsPopescu, A. / Karpay, A. / Israel, D. / Peek Jr., R.M. / Krezel, A.M.
CitationJournal: Proteins / Year: 2005
Title: Helicobacter pylori protein HP0222 belongs to Arc/MetJ family of transcriptional regulators.
Authors: Popescu, A. / Karpay, A. / Israel, D.A. / Peek Jr., R.M. / Krezel, A.M.
History
DepositionAug 19, 2004Deposition site: RCSB / Processing site: RCSB
Revision 1.0Mar 22, 2005Provider: repository / Type: Initial release
Revision 1.1Apr 30, 2008Group: Version format compliance
Revision 1.2Jul 13, 2011Group: Source and taxonomy / Version format compliance
Revision 1.3May 1, 2024Group: Data collection / Database references
Category: chem_comp_atom / chem_comp_bond ...chem_comp_atom / chem_comp_bond / database_2 / pdbx_nmr_software / pdbx_nmr_spectrometer / struct_ref_seq_dif
Item: _database_2.pdbx_DOI / _database_2.pdbx_database_accession ..._database_2.pdbx_DOI / _database_2.pdbx_database_accession / _pdbx_nmr_software.name / _pdbx_nmr_spectrometer.model / _struct_ref_seq_dif.details

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Structure visualization

Structure viewerMolecule:
MolmilJmol/JSmol

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Assembly

Deposited unit
A: hypothetical protein HP0222
B: hypothetical protein HP0222


Theoretical massNumber of molelcules
Total (without water)13,1432
Polymers13,1432
Non-polymers00
Water00
1


  • Idetical with deposited unit
  • defined by author
TypeNameSymmetry operationNumber
identity operation1_555x,y,z1
NMR ensembles
DataCriteria
Number of conformers (submitted / calculated)20 / 80structures with the lowest energy
RepresentativeModel #1closest to the average

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Components

#1: Protein hypothetical protein HP0222


Mass: 6571.399 Da / Num. of mol.: 2
Source method: isolated from a genetically manipulated source
Source: (gene. exp.) Helicobacter pylori (bacteria) / Strain: 26695 / Gene: HP0222 / Plasmid details: pET variant / Plasmid: pET-BNK / Species (production host): Escherichia coli / Production host: Escherichia coli BL21 (bacteria) / Strain (production host): BL21 / References: UniProt: O25010

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Experimental details

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Experiment

ExperimentMethod: SOLUTION NMR
NMR experiment
Conditions-IDExperiment-IDSolution-IDType
1112D TOCSY
1212D NOESY
1313D 15N-separated NOESY
1413D 13C-separated NOESY
NMR detailsText: MRGSHHHHHHGS purification tag was included in protein sequence

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Sample preparation

DetailsContents: HP0222 1mM, 90% H2O, 10% D2O / Solvent system: 90% H2O/10% D2O
Sample conditionsIonic strength: 0 / pH: 4.2 / Pressure: ambient / Temperature: 300 K

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NMR measurement

RadiationProtocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray
Radiation wavelengthRelative weight: 1
NMR spectrometer
TypeManufacturerModelField strength (MHz)Spectrometer-ID
Bruker AVANCEBrukerAVANCE6001
Bruker AVANCEBrukerAVANCE8002

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Processing

NMR software
NameVersionDeveloperClassification
XwinNMR2.6Bruker Inc.processing
Sparky3.106Goddard TD, Kneller DGdata analysis
CYANA1.0.6Guentert P.structure solution
Amber7Case D.refinement
NMR representativeSelection criteria: closest to the average
NMR ensembleConformer selection criteria: structures with the lowest energy
Conformers calculated total number: 80 / Conformers submitted total number: 20

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