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Yorodumi- PDB-1x25: Crystal Structure of a Member of YjgF Family from Sulfolobus Toko... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1x25 | ||||||
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Title | Crystal Structure of a Member of YjgF Family from Sulfolobus Tokodaii (ST0811) | ||||||
Components | Hypothetical UPF0076 protein ST0811 | ||||||
Keywords | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / YJGF-LIKE PROTEIN / ARCHAEA / ST0811 | ||||||
Function / homology | Function and homology information RidA, conserved site / Uncharacterized protein family UPF0076 signature. / RidA family / YjgF/YER057c/UK114 family / Endoribonuclease L-PSP / RutC-like / RutC-like superfamily / 60s Ribosomal Protein L30; Chain: A; / 2-Layer Sandwich / Alpha Beta Similarity search - Domain/homology | ||||||
Biological species | Sulfolobus tokodaii (archaea) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2 Å | ||||||
Authors | Miyakawa, T. / Lee, W.C. / Hatano, K. / Kato, Y. / Sawano, Y. / Miyazono, K. / Nagata, K. / Tanokura, M. | ||||||
Citation | Journal: Proteins / Year: 2006 Title: Crystal structure of the YjgF/YER057c/UK114 family protein from the hyperthermophilic archaeon Sulfolobus tokodaii strain 7 Authors: Miyakawa, T. / Lee, W.C. / Hatano, K. / Kato, Y. / Sawano, Y. / Miyazono, K. / Nagata, K. / Tanokura, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1x25.cif.gz | 65.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1x25.ent.gz | 48.6 KB | Display | PDB format |
PDBx/mmJSON format | 1x25.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/x2/1x25 ftp://data.pdbj.org/pub/pdb/validation_reports/x2/1x25 | HTTPS FTP |
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-Related structure data
Related structure data | 1qd9S S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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2 |
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3 |
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Unit cell |
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Components on special symmetry positions |
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Details | The biological assembly is a trimer generated from a molecule in the asymmetric unit by the operations: -y, x-y, z and -x+y, -x, z. |
-Components
#1: Protein | Mass: 14155.356 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Sulfolobus tokodaii (archaea) / Strain: str. 7 / Gene: ST0811 / Plasmid: pET28a / Production host: Escherichia coli (E. coli) / Strain (production host): Rosetta(DE3) / References: UniProt: Q973T6 #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.33 Å3/Da / Density % sol: 46.7 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 5.3 Details: PEG 10000, Bis-Tris, ammonium acetate, pH 5.3, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU FR-E / Wavelength: 1.5418 Å |
Detector | Type: RIGAKU RAXIS VII / Detector: IMAGE PLATE / Date: Sep 1, 2004 |
Radiation | Monochromator: CONFOCAL MIRROR / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 2→22.051 Å / Num. all: 16882 / Num. obs: 16882 / % possible obs: 99.8 % / Observed criterion σ(I): 5.1 / Redundancy: 6.2 % / Biso Wilson estimate: 17.336 Å2 / Rmerge(I) obs: 0.061 / Rsym value: 0.055 / Net I/σ(I): 9.8 |
Reflection shell | Resolution: 2→2.11 Å / Redundancy: 5.9 % / Rmerge(I) obs: 0.151 / Mean I/σ(I) obs: 5.1 / Num. unique all: 182 / Rsym value: 0.137 / % possible all: 99.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1QD9 Resolution: 2→19.03 Å / Cor.coef. Fo:Fc: 0.96 / Cor.coef. Fo:Fc free: 0.94 / SU B: 2.786 / SU ML: 0.082 / Cross valid method: THROUGHOUT / ESU R: 0.163 / ESU R Free: 0.141 / Stereochemistry target values: MAXIMUM LIKELIHOOD
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.4 Å / Solvent model: BABINET MODEL WITH MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 18.36 Å2
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Refinement step | Cycle: LAST / Resolution: 2→19.03 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2→2.052 Å / Total num. of bins used: 20
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