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Yorodumi- PDB-1w4i: Peripheral-subunit binding domains from mesophilic, thermophilic,... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1w4i | ||||||
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Title | Peripheral-subunit binding domains from mesophilic, thermophilic, and hyperthermophilic bacteria fold by ultrafast, apparently two-state transitions | ||||||
Components | PYRUVATE DEHYDROGENASE E2 | ||||||
Keywords | TRANSFERASE / PERIPHERAL-SUBUNIT BINDING DOMAIN / ULTRAFAST FOLDING / HOMOLOGUES | ||||||
Function / homology | Function and homology information | ||||||
Biological species | PYROBACULUM AEROPHILUM (archaea) | ||||||
Method | SOLUTION NMR | ||||||
Authors | Ferguson, N. / Sharpe, T.D. / Schartau, P.J. / Allen, M.D. / Johnson, C.M. / Sato, S. / Fersht, A.R. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2005 Title: Ultra-Fast Barrier-Limited Folding in the Peripheral Subunit-Binding Domain Family. Authors: Ferguson, N. / Sharpe, T.D. / Schartau, P.J. / Sato, S. / Allen, M.D. / Johnson, C.M. / Rutherford, T.J. / Fersht, A.R. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1w4i.cif.gz | 352.9 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1w4i.ent.gz | 296.9 KB | Display | PDB format |
PDBx/mmJSON format | 1w4i.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/w4/1w4i ftp://data.pdbj.org/pub/pdb/validation_reports/w4/1w4i | HTTPS FTP |
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-Related structure data
Related structure data | 1w4eC 1w4fC 1w4gC 1w4hC 1w4jC 1w4kC 2btgC 2bthC C: citing same article (ref.) |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 6341.314 Da / Num. of mol.: 1 / Fragment: RESIDUES 93-152 Source method: isolated from a genetically manipulated source Source: (gene. exp.) PYROBACULUM AEROPHILUM (archaea) / Plasmid: PRSETA / Production host: ESCHERICHIA COLI (E. coli) / Strain (production host): BL21(DE3) References: UniProt: Q8ZUR6, dihydrolipoyllysine-residue acetyltransferase |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR |
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NMR details | Text: THE STRUCTURE WAS DETERMINED USING TRIPLE-RESONANCE NMR SPECTROSCOPY ON 13C, 15N-LABELED PROTEIN |
-Sample preparation
Details | Contents: 3MM SAMPLE, 95% H2O/5% D2O |
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Sample conditions | Ionic strength: 100 / pH: 6.2 / Pressure: 1.0 atm / Temperature: 298.0 K |
-NMR measurement
NMR spectrometer | Type: Bruker DRX / Manufacturer: Bruker / Model: DRX / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE JRNL CITATION ABOVE. | ||||||||||||
NMR ensemble | Conformer selection criteria: NO VIOLATION > 0.25 / Conformers calculated total number: 21 / Conformers submitted total number: 20 |