ジャーナル: J.Biol.Chem. / 年: 2004 タイトル: Structural Determinants of Substrate Specificity in Family 1 Beta-Glucosidases: Novel Insights from the Crystal Structure of Sorghum Dhurrinase-1, a Plant Beta-Glucosidase with Strict ...タイトル: Structural Determinants of Substrate Specificity in Family 1 Beta-Glucosidases: Novel Insights from the Crystal Structure of Sorghum Dhurrinase-1, a Plant Beta-Glucosidase with Strict Specificity, in Complex with its Natural Substrate 著者: Verdoucq, L. / Moriniere, J. / Bevan, D.R. / Esen, A. / Vasella, A. / Henrissat, B. / Czjzek, M.
SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA BA" IN EACH CHAIN ON SHEET RECORDS ... SHEET DETERMINATION METHOD: DSSP THE SHEETS PRESENTED AS "AA BA" IN EACH CHAIN ON SHEET RECORDS BELOW IS ACTUALLY AN 9-STRANDED BARREL THIS IS REPRESENTED BY A 10-STRANDED SHEET IN WHICH THE FIRST AND LAST STRANDS ARE IDENTICAL.
ENGINEERED MUTATION IN CHAIN A AND B, GLU 245 TO ASP CATALYTIC ACTIVITY: HYDROLYSIS OF TERMINAL, ...ENGINEERED MUTATION IN CHAIN A AND B, GLU 245 TO ASP CATALYTIC ACTIVITY: HYDROLYSIS OF TERMINAL, NON-REDUCING BETA-D- GLUCOSE RESIDUES WITH RELEASE OF BETA-D-GLUCOSE.
解像度: 1.9→29.75 Å / Cor.coef. Fo:Fc: 0.962 / Cor.coef. Fo:Fc free: 0.949 / SU B: 2.826 / SU ML: 0.078 / 交差検証法: THROUGHOUT / ESU R: 0.138 / ESU R Free: 0.126 / 立体化学のターゲット値: MAXIMUM LIKELIHOOD / 詳細: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
Rfactor
反射数
%反射
Selection details
Rfree
0.205
4604
5 %
RANDOM
Rwork
0.173
-
-
-
obs
0.175
87352
100 %
-
溶媒の処理
イオンプローブ半径: 0.8 Å / 減衰半径: 0.8 Å / VDWプローブ半径: 1.4 Å / 溶媒モデル: BABINET MODEL WITH MASK