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Open data
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Basic information
Entry | Database: PDB / ID: 1uz8 | |||||||||
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Title | anti-Lewis X Fab fragment in complex with Lewis X | |||||||||
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![]() | IMMUNE SYSTEM / ANTIBODY / ANTI-CARBOHYDRATE | |||||||||
Function / homology | Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta![]() | |||||||||
Biological species | ![]() ![]() | |||||||||
Method | ![]() ![]() ![]() | |||||||||
![]() | Van Roon, A.M.M. / Pannu, N.S. / De Vrind, J.P.M. / Hokke, C.H. / Deelder, A.M. / Van Der marel, G.A. / Van Boom, J.H. / Abrahams, J.P. | |||||||||
![]() | ![]() Title: Structure of an Anti-Lewis X Fab Fragment in Complex with its Lewis X Antigen Authors: Van Roon, A.M.M. / Pannu, N.S. / De Vrind, J.P.M. / Van Der Marel, G.A. / Van Boom, J.H. / Hokke, C.H. / Deelder, A.M. / Abrahams, J.P. | |||||||||
History |
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Remark 700 | SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN ... SHEET THE SHEET STRUCTURE OF THIS MOLECULE IS BIFURCATED. IN ORDER TO REPRESENT THIS FEATURE IN THE SHEET RECORDS BELOW, TWO SHEETS ARE DEFINED. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 187.2 KB | Display | ![]() |
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PDB format | ![]() | 148.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 1.3 MB | Display | ![]() |
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Full document | ![]() | 1.3 MB | Display | |
Data in XML | ![]() | 19 KB | Display | |
Data in CIF | ![]() | 30 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1uz6SC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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3 |
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Unit cell |
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Noncrystallographic symmetry (NCS) | NCS oper: (Code: given Matrix: (0.3981, -0.64951, 0.64781), Vector: |
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Components
#1: Antibody | Mass: 24065.650 Da / Num. of mol.: 2 / Fragment: FAB FRAGMENT LIGHT CHAIN, RESIDUES 1-212 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #2: Antibody | Mass: 22933.863 Da / Num. of mol.: 2 / Fragment: FAB FRAGMENT HEAVY CHAIN, RESIDUES 1-213 / Source method: isolated from a natural source / Source: (natural) ![]() ![]() #3: Polysaccharide | Source method: isolated from a genetically manipulated source #4: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.5 Å3/Da / Density % sol: 49.9 % |
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Crystal grow | pH: 8 / Details: 14 % PEG 3350, 50 MM TRIS PH 8 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: ADSC CCD / Detector: CCD / Date: Apr 25, 2003 / Details: MIRRORS |
Radiation | Monochromator: DIAMOND (111), GE(220) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.93 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→45.175 Å / Num. obs: 81582 / % possible obs: 92.6 % / Observed criterion σ(I): 2 / Redundancy: 4.1 % / Rmerge(I) obs: 0.094 / Net I/σ(I): 11.1 |
Reflection shell | Resolution: 1.8→1.86 Å / Redundancy: 3.2 % / Rmerge(I) obs: 0.386 / Mean I/σ(I) obs: 2.2 / % possible all: 72 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1UZ6 Resolution: 1.8→91.29 Å / Cor.coef. Fo:Fc: 0.951 / Cor.coef. Fo:Fc free: 0.931 / SU B: 7.144 / SU ML: 0.109 / TLS residual ADP flag: LIKELY RESIDUAL / Cross valid method: THROUGHOUT / ESU R: 0.148 / ESU R Free: 0.139 / Stereochemistry target values: MAXIMUM LIKELIHOOD / Details: HYDROGENS HAVE BEEN ADDED IN THE RIDING POSITIONS
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Solvent computation | Ion probe radii: 0.8 Å / Shrinkage radii: 0.8 Å / VDW probe radii: 1.2 Å / Solvent model: BABINET MODEL PLUS MASK | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 39.17 Å2
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Refinement step | Cycle: LAST / Resolution: 1.8→91.29 Å
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Refine LS restraints |
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