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Yorodumi- PDB-1uwd: NMR STRUCTURE OF A PROTEIN WITH UNKNOWN FUNCTION FROM THERMOTOGA ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1uwd | ||||||
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Title | NMR STRUCTURE OF A PROTEIN WITH UNKNOWN FUNCTION FROM THERMOTOGA MARITIMA (TM0487), WHICH BELONGS TO THE DUF59 FAMILY. | ||||||
Components | HYPOTHETICAL PROTEIN TM0487 | ||||||
Keywords | BIOSYNTHETIC PROTEIN / SIMILAR TO PAAD PROTEIN / ALPHA/BETA FOLD / STRUCTURAL GENOMICS / JOINT CENTER FOR STRUCTURAL GENOMICS / JCSG / PROTEIN STRUCTURE INITIATIVE / PSI | ||||||
Function / homology | Fe-S cluster assembly (FSCA) / MIP18 family-like / Iron-sulfur cluster assembly protein / Fe-S cluster assembly domain superfamily / GMP Synthetase; Chain A, domain 3 / 2-Layer Sandwich / Alpha Beta / MIP18 family-like domain-containing protein Function and homology information | ||||||
Biological species | THERMOTOGA MARITIMA (bacteria) | ||||||
Method | SOLUTION NMR / distance geometry | ||||||
Authors | Almeida, M.S. / Peti, W. / Herrmann, T. / Wuthrich, K. | ||||||
Citation | Journal: Protein Sci. / Year: 2005 Title: NMR Structure of the Conserved Hypothetical Protein Tm0487 from Thermotoga Maritima: Implications for 216 Homologous Duf59 Proteins. Authors: Almeida, M.S. / Herrmann, T. / Peti, W. / Wilson, I.A. / Wuthrich, K. #1: Journal: J.Biomol.NMR / Year: 2004 Title: 1H-, 13C- and 15N-NMR Assignment of the Conserved Hypothetical Protein Tm0487 from Thermotoga Maritima Authors: Almeida, M.S. / Peti, W. / Wuthrich, K. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1uwd.cif.gz | 631 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1uwd.ent.gz | 526.2 KB | Display | PDB format |
PDBx/mmJSON format | 1uwd.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1uwd_validation.pdf.gz | 355.5 KB | Display | wwPDB validaton report |
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Full document | 1uwd_full_validation.pdf.gz | 465.5 KB | Display | |
Data in XML | 1uwd_validation.xml.gz | 28.4 KB | Display | |
Data in CIF | 1uwd_validation.cif.gz | 51 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/uw/1uwd ftp://data.pdbj.org/pub/pdb/validation_reports/uw/1uwd | HTTPS FTP |
-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 11517.340 Da / Num. of mol.: 1 / Fragment: RESIDUES 2-104 Source method: isolated from a genetically manipulated source Source: (gene. exp.) THERMOTOGA MARITIMA (bacteria) / Production host: ESCHERICHIA COLI (E. coli) / References: UniProt: Q9WYV7 |
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Sequence details | MET 1 AND PRO 2 ARE NOT INCLUDED IN THESE STRUCTURES |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||
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NMR experiment |
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NMR details | Text: THE SAMPLE WAS 13C AND 15N-LABELED. |
-Sample preparation
Details | Contents: 90% H2O/10% D2O, 20MM SODIUM PHOSPHATE |
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Sample conditions | pH: 6.0 / Pressure: 1 atm / Temperature: 313 K |
-NMR measurement
NMR spectrometer | Type: Bruker AVANCE / Manufacturer: Bruker / Model: AVANCE / Field strength: 900 MHz |
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-Processing
NMR software |
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Refinement | Method: distance geometry / Software ordinal: 1 Details: THESE STRUCTURES WERE ENERGY- MINIMIZED IN A WATER SHELL USING THE AMBER FORCE FIELD. 20 LOWEST ENERGY NMR STRUCTURES. THESE STRUCTURES WERE FITTED BY MOLMOL. | ||||||||||||
NMR ensemble | Conformers calculated total number: 100 / Conformers submitted total number: 20 |