[English] 日本語
![](img/lk-miru.gif)
- PDB-1tys: WATER-MEDIATED SUBSTRATE(SLASH)PRODUCT DISCRIMINATION: THE PRODUC... -
+
Open data
-
Basic information
Entry | Database: PDB / ID: 1tys | ||||||
---|---|---|---|---|---|---|---|
Title | WATER-MEDIATED SUBSTRATE(SLASH)PRODUCT DISCRIMINATION: THE PRODUCT COMPLEX OF THYMIDYLATE SYNTHASE AT 1.83 ANGSTROMS | ||||||
![]() | THYMIDYLATE SYNTHASE | ||||||
![]() | TRANSFERASE / METHYLTRANSFERASE | ||||||
Function / homology | ![]() thymidylate synthase / thymidylate synthase activity / dTMP biosynthetic process / dTTP biosynthetic process / response to radiation / regulation of translation / methylation / magnesium ion binding / protein homodimerization activity / RNA binding ...thymidylate synthase / thymidylate synthase activity / dTMP biosynthetic process / dTTP biosynthetic process / response to radiation / regulation of translation / methylation / magnesium ion binding / protein homodimerization activity / RNA binding / cytoplasm / cytosol Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | Fauman, E. / Rutenber, E. / Stroud, R. | ||||||
![]() | ![]() Title: Water-mediated substrate/product discrimination: the product complex of thymidylate synthase at 1.83 A. Authors: Fauman, E.B. / Rutenber, E.E. / Maley, G.F. / Maley, F. / Stroud, R.M. | ||||||
History |
|
-
Structure visualization
Structure viewer | Molecule: ![]() ![]() |
---|
-
Downloads & links
-
Download
PDBx/mmCIF format | ![]() | 72.3 KB | Display | ![]() |
---|---|---|---|---|
PDB format | ![]() | 53 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 501 KB | Display | ![]() |
---|---|---|---|---|
Full document | ![]() | 505.8 KB | Display | |
Data in XML | ![]() | 7.8 KB | Display | |
Data in CIF | ![]() | 11.8 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
---|
-
Links
-
Assembly
Deposited unit | ![]()
| |||||||||
---|---|---|---|---|---|---|---|---|---|---|
1 | ![]()
| |||||||||
Unit cell |
| |||||||||
Atom site foot note | 1: RESIDUE CXM 1 IS A FORMATE GROUP BOUND TO N ATOM OF METHIONINE. | |||||||||
Components on special symmetry positions |
|
-
Components
#1: Protein | Mass: 30543.600 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() ![]() References: UniProt: P00470, UniProt: P0A884*PLUS, thymidylate synthase |
---|---|
#2: Chemical | ChemComp-TMP / |
#3: Chemical | ChemComp-DHF / |
#4: Water | ChemComp-HOH / |
Compound details | RESIDUE CXM 1 IS A FORMATE GROUP BOUND TO N ATOM OF METHIONINE |
-Experimental details
-Experiment
Experiment | Method: ![]() |
---|
-
Sample preparation
Crystal | Density Matthews: 2.82 Å3/Da / Density % sol: 56.32 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Crystal grow | *PLUS Temperature: 23 ℃ / Method: vapor diffusion, hanging drop / pH: 8 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
|
-Data collection
Radiation | Scattering type: x-ray |
---|---|
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 1.8→15 Å / Num. obs: 30830 |
Reflection | *PLUS Highest resolution: 1.8 Å / Lowest resolution: 9999 Å / Num. obs: 30830 / Rmerge(I) obs: 0.094 |
Reflection shell | *PLUS Highest resolution: 1.8 Å / Lowest resolution: 1.9 Å / Num. possible: 4845 / Num. unique obs: 3117 / Rmerge(I) obs: 0.312 |
-
Processing
Software |
| ||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Refinement | Resolution: 1.8→7 Å / σ(F): 0 /
| ||||||||||||
Refinement step | Cycle: LAST / Resolution: 1.8→7 Å
| ||||||||||||
Software | *PLUS Name: ![]() | ||||||||||||
Refinement | *PLUS Num. reflection all: 30830 / Rfactor obs: 0.183 / Rfactor Rwork: 0.183 | ||||||||||||
Solvent computation | *PLUS | ||||||||||||
Displacement parameters | *PLUS |