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Open data
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Basic information
Entry | Database: PDB / ID: 1trh | ||||||
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Title | TWO CONFORMATIONAL STATES OF CANDIDA RUGOSA LIPASE | ||||||
![]() | LIPASE | ||||||
![]() | HYDROLASE(CARBOXYLIC ESTERASE) | ||||||
Function / homology | ![]() triacylglycerol lipase / triacylglycerol lipase activity / lipid catabolic process Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | ![]() | ||||||
![]() | Grochulski, P. / Cygler, M. | ||||||
![]() | ![]() Title: Two conformational states of Candida rugosa lipase. Authors: Grochulski, P. / Li, Y. / Schrag, J.D. / Cygler, M. #1: ![]() Title: Insights Into Interfacial Activation from an Open Structure of Candida Rugosa Lipase Authors: Grochulski, P. / Li, Y. / Schrag, J.D. / Bouthillier, F. / Smith, P. / Harrison, D. / Rubin, B. / Cygler, M. #2: ![]() Title: Cloning and Analysis of Candida Cylindracea Lipase Sequences Authors: Lotti, M. / Grandori, R. / Fusetti, F. / Longhi, S. / Brocca, S. / Tramontano, A. #3: ![]() Title: The Codon Cug is Read as Serine in an Asporogenic Yeast Candida Cylindracea Authors: Kawaguchi, Y. / Honda, H. / Taniguchi-Morimura, J. / Iwasaki, S. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 114.8 KB | Display | ![]() |
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PDB format | ![]() | 91.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 385.1 KB | Display | ![]() |
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Full document | ![]() | 392.7 KB | Display | |
Data in XML | ![]() | 11.9 KB | Display | |
Data in CIF | ![]() | 19.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO 92 / 2: CIS PROLINE - PRO 390 |
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Components
#1: Protein | Mass: 57133.859 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() | ||
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#2: Sugar | #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.92 Å3/Da / Density % sol: 57.87 % | ||||||||||||||||||||
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Crystal grow | *PLUS Method: vapor diffusion, hanging drop / Details: macroseeding / pH: 6.5 | ||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Reflection | *PLUS Highest resolution: 2.1 Å / Num. obs: 30450 / % possible obs: 81 % / Observed criterion σ(I): 1 / Num. measured all: 52852 / Rmerge(I) obs: 0.064 |
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Reflection shell | *PLUS Highest resolution: 2.1 Å / Lowest resolution: 2.25 Å / % possible obs: 61 % |
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Processing
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Refinement | Rfactor Rwork: 0.148 / Rfactor obs: 0.148 / Highest resolution: 2.1 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 2.1 Å
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Refine LS restraints |
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Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.1 Å / Lowest resolution: 8 Å / Num. reflection obs: 20701 / σ(I): 2 / Rfactor all: 0.167 / Rfactor obs: 0.148 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 22.9 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: x_angle_d / Dev ideal: 2.7 |