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Yorodumi- PDB-1tks: Crystal structure of 3,4-Dihydroxy-2-butanone 4-phosphate Synthas... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1tks | ||||||
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Title | Crystal structure of 3,4-Dihydroxy-2-butanone 4-phosphate Synthase of Candida albicans | ||||||
Components | 3,4-dihydroxy-2-butanone 4-phosphate synthase | ||||||
Keywords | ISOMERASE / Candida albicans / riboflavin biosynthesis / 3 / 4-dihydroxy-2-butanone 4-phosphate synthase / crystal structure synthetic gene | ||||||
Function / homology | Function and homology information 3,4-dihydroxy-2-butanone-4-phosphate synthase / 3,4-dihydroxy-2-butanone-4-phosphate synthase activity / riboflavin biosynthetic process / metal ion binding Similarity search - Function | ||||||
Biological species | Candida albicans (yeast) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.6 Å | ||||||
Authors | Echt, S. / Bauer, S. / Steinbacher, S. / Huber, R. / Bacher, A. / Fischer, M. | ||||||
Citation | Journal: J.Mol.Biol. / Year: 2004 Title: Potential anti-infective targets in pathogenic yeasts: structure and properties of 3,4-dihydroxy-2-butanone 4-phosphate synthase of Candida albicans. Authors: Echt, S. / Bauer, S. / Steinbacher, S. / Huber, R. / Bacher, A. / Fischer, M. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1tks.cif.gz | 101.5 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1tks.ent.gz | 76.5 KB | Display | PDB format |
PDBx/mmJSON format | 1tks.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/tk/1tks ftp://data.pdbj.org/pub/pdb/validation_reports/tk/1tks | HTTPS FTP |
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-Related structure data
Related structure data | 1tkuC 1g57S C: citing same article (ref.) S: Starting model for refinement |
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Similar structure data |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 22684.982 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Candida albicans (yeast) / Strain: SC5314 / Gene: CARib3 / Plasmid: pNCO-CARib3-syn / Production host: Escherichia coli (E. coli) / Strain (production host): XL1 References: GenBank: 46438026, UniProt: Q5A3V6*PLUS, Isomerases; Intramolecular transferases; Transferring other groups #2: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.18 Å3/Da / Density % sol: 43 % |
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Crystal grow | Temperature: 293 K / Method: vapor diffusion, sitting drop / pH: 5 Details: Na-Citrat, PEG8000, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU RU200 / Wavelength: 1.542 Å |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Jul 3, 2003 |
Radiation | Monochromator: graphite / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.542 Å / Relative weight: 1 |
Reflection | Resolution: 1.6→20 Å / Num. obs: 43264 / % possible obs: 84.4 % / Redundancy: 3.8 % / Rmerge(I) obs: 0.059 / Net I/σ(I): 27.7 |
Reflection shell | Resolution: 1.6→1.66 Å / Rmerge(I) obs: 0.221 / % possible all: 81.9 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PDB ENTRY 1G57 Resolution: 1.6→20 Å
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Refinement step | Cycle: LAST / Resolution: 1.6→20 Å
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LS refinement shell | Resolution: 1.6→1.66 Å
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