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Yorodumi- PDB-1te7: Solution NMR Structure of Protein yqfB from Escherichia coli. Nor... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1te7 | |||||||||
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Title | Solution NMR Structure of Protein yqfB from Escherichia coli. Northeast Structural Genomics Consortium Target ET99 | |||||||||
Components | Hypothetical UPF0267 protein yqfB | |||||||||
Keywords | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / ALPHA + BETA / PSI / Protein Structure Initiative / Northeast Structural Genomics Consortium / NESG | |||||||||
Function / homology | Function and homology information N4-acetylcytidine amidohydrolase / hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines / cytosol Similarity search - Function | |||||||||
Biological species | Escherichia coli (E. coli) | |||||||||
Method | SOLUTION NMR / torsion angle dynamics with DYANA | |||||||||
Authors | Atreya, H.S. / Shen, Y. / Yee, A. / Arrowsmith, C. / Szyperski, T. / Northeast Structural Genomics Consortium (NESG) | |||||||||
Citation | Journal: J.Am.Chem.Soc. / Year: 2005 Title: G-Matrix Fourier Transform NOESY-Based Protocol for High-Quality Protein Structure Determination Authors: Shen, Y. / Atreya, H.S. / Liu, G. / Szyperski, T. | |||||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1te7.cif.gz | 643.1 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1te7.ent.gz | 535 KB | Display | PDB format |
PDBx/mmJSON format | 1te7.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/te/1te7 ftp://data.pdbj.org/pub/pdb/validation_reports/te/1te7 | HTTPS FTP |
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-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein | Mass: 11918.377 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Escherichia coli (E. coli) / Gene: YQFB / Production host: Escherichia coli (E. coli) / References: UniProt: P67603 |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details | Contents: 1mM U-13C,15N YqfB,25 mM Na phosphate, 400 mM NaCl, 1 mM DTT, 20 mM ZnCl2, 0.01% NaN3, 90% H2O, 10% D2O Solvent system: 90% H2O/10% D2O |
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Sample conditions | pH: 6.5 / Temperature units: K |
-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M |
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Radiation wavelength | Relative weight: 1 |
NMR spectrometer | Type: Varian INOVA / Manufacturer: Varian / Model: INOVA / Field strength: 600 MHz |
-Processing
NMR software |
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Refinement | Method: torsion angle dynamics with DYANA / Software ordinal: 1 | ||||||||||||||||||||
NMR representative | Selection criteria: fewest violations,lowest energy | ||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the least restraint violations,structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 20 |