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- PDB-1krx: SOLUTION STRUCTURE OF BERYLLOFLUORIDE-ACTIVATED NTRC RECEIVER DOM... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1krx | ||||||
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Title | SOLUTION STRUCTURE OF BERYLLOFLUORIDE-ACTIVATED NTRC RECEIVER DOMAIN: MODEL STRUCTURES INCORPORATING ACTIVE SITE CONTACTS | ||||||
![]() | NITROGEN REGULATION PROTEIN NR(I) | ||||||
![]() | SIGNALING PROTEIN / two component signal transduction / receiver domain / BeF3 / phosphorylation / Bacterial nitrogen regulatory protein | ||||||
Function / homology | ![]() regulation of nitrogen utilization / nitrogen fixation / phosphorelay response regulator activity / sequence-specific DNA binding / regulation of DNA-templated transcription / ATP hydrolysis activity / ATP binding / cytoplasm Similarity search - Function | ||||||
Biological species | ![]() | ||||||
Method | SOLUTION NMR / distance geometry, torsion angle dynamics, simulated annealing | ||||||
![]() | Hastings, C.A. / Lee, S.-Y. / Cho, H.S. / Yan, D. / Kustu, S. / Wemmer, D.E. | ||||||
![]() | ![]() Title: High-Resolution Solution Structure of the Beryllofluoride-Activated NtrC Receiver Domain Authors: Hastings, C.A. / Lee, S.-Y. / Cho, H.S. / Yan, D. / Kustu, S. / Wemmer, D.E. #1: ![]() Title: NMR STRUCTURE OF ACTIVATED CHEY Authors: Cho, H.S. / Lee, S.-Y. / Yan, D. / Pan, X. / Parkinson, J.S. / Kustu, S. / Wemmer, D.E. / Pelton, J.G. #2: ![]() Title: CRYSTAL STRUCTURE OF AN ACTIVATED RESPONSE REGULATOR BOUND TO ITS TARGET Authors: Lee, S.-Y. / Cho, H.S. / Pelton, J.G. / Yan, D. / Henderson, R.K. / King, D.S. / Huang, L. / Kustu, S. / Berry, E.A. / Wemmer, D.E. | ||||||
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 968.9 KB | Display | ![]() |
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PDB format | ![]() | 806.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 359.6 KB | Display | ![]() |
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Full document | ![]() | 630.3 KB | Display | |
Data in XML | ![]() | 103.1 KB | Display | |
Data in CIF | ![]() | 134.3 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
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Assembly
Deposited unit | ![]()
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NMR ensembles |
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Components
#1: Protein | Mass: 13636.604 Da / Num. of mol.: 1 Fragment: N-terminal domain: Receiver domain, Residues 1-124 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() ![]() |
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#2: Chemical | ChemComp-BEF / |
-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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NMR details | Text: Coordinates for the 26 structures of the model derived from NMR constraints and 3 active site arrangement constraints inferred from similarity to CheY |
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Sample preparation
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Sample conditions |
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-NMR measurement
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M | |||||||||||||||
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Radiation wavelength | Relative weight: 1 | |||||||||||||||
NMR spectrometer |
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Processing
NMR software |
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Refinement | Method: distance geometry, torsion angle dynamics, simulated annealing Software ordinal: 1 Details: 3 additional constraints from crystal structure of BeF3-activated CheY were used for structure calculation. | ||||||||||||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 100 / Conformers submitted total number: 26 |