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Yorodumi- PDB-1fe3: CRYSTAL STRUCTURE OF HUMAN BRAIN FATTY ACID BINDING PROTEIN OLEIC ACID -
+Open data
-Basic information
Entry | Database: PDB / ID: 1fe3 | ||||||
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Title | CRYSTAL STRUCTURE OF HUMAN BRAIN FATTY ACID BINDING PROTEIN OLEIC ACID | ||||||
Components | FATTY ACID-BINDING PROTEIN, BRAIN | ||||||
Keywords | LIPID BINDING PROTEIN / OA / BLBP / LIPID-BINDING PROTEIN | ||||||
Function / homology | Function and homology information NOTCH3 Intracellular Domain Regulates Transcription / Triglyceride catabolism / fatty acid transport / epithelial cell proliferation / fatty acid binding / nervous system development / negative regulation of cell population proliferation / lipid binding / nucleus / cytosol Similarity search - Function | ||||||
Biological species | Homo sapiens (human) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 2.8 Å | ||||||
Authors | Balendiran, G.K. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2000 Title: Crystal structure and thermodynamic analysis of human brain fatty acid-binding protein. Authors: Balendiran, G.K. / Schnutgen, F. / Scapin, G. / Borchers, T. / Xhong, N. / Lim, K. / Godbout, R. / Spener, F. / Sacchettini, J.C. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1fe3.cif.gz | 37.2 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1fe3.ent.gz | 25.3 KB | Display | PDB format |
PDBx/mmJSON format | 1fe3.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/fe/1fe3 ftp://data.pdbj.org/pub/pdb/validation_reports/fe/1fe3 | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 14776.702 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Homo sapiens (human) / References: UniProt: O15540 |
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#2: Chemical | ChemComp-OLA / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 4 Å3/Da / Density % sol: 69.22 % | |||||||||||||||
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Crystal grow | Method: vapor diffusion, hanging drop / Details: VAPOR DIFFUSION, HANGING DROP | |||||||||||||||
Crystal grow | *PLUS pH: 8.5 | |||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction source | Source: ROTATING ANODE |
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Detector | Type: SIEMENS / Detector: AREA DETECTOR |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Relative weight: 1 |
Reflection | Resolution: 2.8→20 Å / Num. all: 11489 / Num. obs: 11489 / % possible obs: 82.5 % / Rsym value: 0.072 |
Reflection | *PLUS Lowest resolution: 15 Å / Num. obs: 5444 / Num. measured all: 11489 / Rmerge(I) obs: 0.072 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 2.8→15 Å
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Refinement step | Cycle: LAST / Resolution: 2.8→15 Å
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Software | *PLUS Name: TNT / Classification: refinement | ||||||||||||||||||||||||||||||
Refinement | *PLUS Highest resolution: 2.8 Å / Lowest resolution: 20 Å / Rfactor obs: 0.19 | ||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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