+Open data
-Basic information
Entry | Database: PDB / ID: 1wad | ||||||
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Title | CYTOCHROME C3 WITH 4 HEME GROUPS AND ONE CALCIUM ION | ||||||
Components | CYTOCHROME C3 | ||||||
Keywords | ELECTRON TRANSPORT / CALCIUM BINDING | ||||||
Function / homology | Function and homology information anaerobic respiration / electron transfer activity / heme binding / metal ion binding Similarity search - Function | ||||||
Biological species | Desulfovibrio gigas (bacteria) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / Resolution: 1.8 Å | ||||||
Authors | Matias, P.M. / Morais, J. / Coelho, R. / Carrondo, M.A. / Wilson, K. / Dauter, Z. / Sieker, L. | ||||||
Citation | Journal: Protein Sci. / Year: 1996 Title: Cytochrome c3 from Desulfovibrio gigas: crystal structure at 1.8 A resolution and evidence for a specific calcium-binding site. Authors: Matias, P.M. / Morais, J. / Coelho, R. / Carrondo, M.A. / Wilson, K. / Dauter, Z. / Sieker, L. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1wad.cif.gz | 36.3 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1wad.ent.gz | 28.3 KB | Display | PDB format |
PDBx/mmJSON format | 1wad.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/wa/1wad ftp://data.pdbj.org/pub/pdb/validation_reports/wa/1wad | HTTPS FTP |
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-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 12003.509 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Desulfovibrio gigas (bacteria) / References: UniProt: P00133 | ||
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#2: Chemical | ChemComp-CA / | ||
#3: Chemical | ChemComp-HEM / #4: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION |
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-Sample preparation
Crystal | Density Matthews: 2.25 Å3/Da / Density % sol: 40.9 % | ||||||||||||||||||||||||||||||
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Crystal grow | pH: 6.5 / Details: pH 6.5 | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Temperature: 10 ℃ / Method: batch method | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 293 K |
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Diffraction source | Source: SYNCHROTRON / Site: EMBL/DESY, Hamburg / Beamline: X11 / Wavelength: 0.928 |
Detector | Type: MAR scanner 180 mm plate / Detector: IMAGE PLATE / Date: Oct 1, 1994 / Details: FOCUSSING MONOCHROMATOR A |
Radiation | Monochromator: GE(111) / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.928 Å / Relative weight: 1 |
Reflection | Resolution: 1.8→36 Å / Num. obs: 10315 / % possible obs: 98.3 % / Observed criterion σ(I): 0 / Redundancy: 4.3 % / Rmerge(I) obs: 0.059 |
Reflection | *PLUS Num. measured all: 44409 |
Reflection shell | *PLUS Highest resolution: 1.8 Å / Lowest resolution: 1.83 Å / % possible obs: 96 % / Rmerge(I) obs: 0.188 |
-Processing
Software |
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Refinement | Resolution: 1.8→36 Å / σ(F): 1
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Refinement step | Cycle: LAST / Resolution: 1.8→36 Å
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