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- PDB-1t04: Three dimensional structure of a humanized anti-IFN-Gamma Fab in ... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1t04 | |||||||||
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Title | Three dimensional structure of a humanized anti-IFN-Gamma Fab in C2 space group | |||||||||
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![]() | IMMUNE SYSTEM / Antibody Engineering / Humanized and Chimeric Antibody / FAB / three-dimensional structure / Gamma-interferon | |||||||||
Function / homology | Immunoglobulins / Immunoglobulin-like / Sandwich / Mainly Beta / : ![]() | |||||||||
Biological species | ![]() | |||||||||
Method | ![]() ![]() | |||||||||
![]() | Bourne, P.C. / Terzyan, S.S. / Cloud, G. / Landolfi, N.F. / Vasquez, M. / Edmundson, A.B. | |||||||||
![]() | ![]() Title: Three-dimensional structures of a humanized anti-IFN-gamma Fab (HuZAF) in two crystal forms. Authors: Bourne, P.C. / Terzyan, S.S. / Cloud, G. / Landolfi, N.F. / Vasquez, M. / Edmundson, A.B. | |||||||||
History |
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Remark 999 | SEQUENCE THERE IS NO SEQUENCE DATABASE REFERENCE FOR THIS ENTRY. THE SEQUENCE WAS DETERMINED IN THE ...SEQUENCE THERE IS NO SEQUENCE DATABASE REFERENCE FOR THIS ENTRY. THE SEQUENCE WAS DETERMINED IN THE PROTEIN DESIGN LABORATORY. |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 173.5 KB | Display | ![]() |
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PDB format | ![]() | 139 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 451.7 KB | Display | ![]() |
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Full document | ![]() | 477 KB | Display | |
Data in XML | ![]() | 34.1 KB | Display | |
Data in CIF | ![]() | 46.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 1t3fSC S: Starting model for refinement C: citing same article ( |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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2 | ![]()
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Unit cell |
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Components
#1: Antibody | Mass: 23430.879 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: The source is engineered human constant and framework regions with CDRs from mouse. Source: (gene. exp.) ![]() #2: Antibody | Mass: 23377.156 Da / Num. of mol.: 2 Source method: isolated from a genetically manipulated source Details: The source is engineered human constant and framework regions with CDRs from mouse. Source: (gene. exp.) ![]() #3: Water | ChemComp-HOH / | |
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-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.7 Å3/Da / Density % sol: 46 % |
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Crystal grow | Temperature: 288 K / pH: 5.6 Details: PEG8000, (NH4)2SO4, MgCl2, MES, pH 5.60, VAPOR DIFFUSION, SITTING DROP, temperature 288K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Apr 20, 2003 / Details: OSMIC BLUE OPTICS |
Radiation | Monochromator: OSMIC BLUE OPTICS / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.54 Å / Relative weight: 1 |
Reflection | Resolution: 3→25 Å / Num. obs: 19324 / % possible obs: 97.1 % / Observed criterion σ(I): -3 / Redundancy: 2.49 % / Biso Wilson estimate: 51.72 Å2 / Rmerge(I) obs: 0.146 / Net I/σ(I): 6.14 |
Reflection shell | Resolution: 3→3.11 Å / Rmerge(I) obs: 0.387 / Mean I/σ(I) obs: 2.27 / % possible all: 96.9 |
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Processing
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Refinement | Method to determine structure: ![]() Starting model: PDB ENTRY 1T3F Resolution: 3→25 Å / Isotropic thermal model: OVERALL ANISOTROPIC / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: ENGH & HUBER
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Solvent computation | Solvent model: CNS / Bsol: 31.82 Å2 / ksol: 0.36 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 28.27 Å2
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Refinement step | Cycle: LAST / Resolution: 3→25 Å
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Refine LS restraints |
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Xplor file |
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