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- PDB-1ssc: THE 1.6 ANGSTROMS STRUCTURE OF A SEMISYNTHETIC RIBONUCLEASE CRYST... -
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Open data
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Basic information
Entry | Database: PDB / ID: 1ssc | ||||||
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Title | THE 1.6 ANGSTROMS STRUCTURE OF A SEMISYNTHETIC RIBONUCLEASE CRYSTALLIZED FROM AQUEOUS ETHANOL. COMPARISON WITH CRYSTALS FROM SALT SOLUTIONS AND WITH RNASE A FROM AQUEOUS ALCOHOL SOLUTIONS | ||||||
![]() | (RIBONUCLEASE A) x 2 | ||||||
![]() | ENDONUCLEASE | ||||||
Function / homology | ![]() pancreatic ribonuclease / ribonuclease A activity / RNA nuclease activity / nucleic acid binding / lyase activity / defense response to Gram-positive bacterium / extracellular region Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() | ||||||
![]() | De Mel, V.S.J. / Doscher, M.S. / Martin, P.D. / Rodier, F. / Edwards, B.F.P. | ||||||
![]() | ![]() Title: 1.6 A structure of semisynthetic ribonuclease crystallized from aqueous ethanol. Comparison with crystals from salt solutions and with ribonuclease A from aqueous alcohol solutions. Authors: de Mel, S.J. / Doscher, M.S. / Martin, P.D. / Rodier, F. / Edwards, B.F. #1: ![]() Title: Structural Changes that Accompany the Reduced Catalytic Efficiency of Two Semisynthetic Ribonuclease Analogs Authors: De Mel, V.S.J. / Martin, P.D. / Doscher, M.S. / Edwards, B.F.P. #2: ![]() Title: The Refined Crystal Structure of Fully Active Semisynthetic Ribonuclease At 1.8-A Resolution. Authors: Martin, P.D. / Doscher, M.S. / Edwards, B.F.P. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 32.9 KB | Display | ![]() |
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PDB format | ![]() | 25.5 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 384 KB | Display | ![]() |
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Full document | ![]() | 389.2 KB | Display | |
Data in XML | ![]() | 4.8 KB | Display | |
Data in CIF | ![]() | 7.1 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 |
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Unit cell |
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Atom site foot note | 1: CIS PROLINE - PRO A 93 |
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Components
#1: Protein | Mass: 12380.861 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
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#2: Protein/peptide | Mass: 1231.375 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) ![]() ![]() |
#3: Chemical | ChemComp-PO4 / |
#4: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 2.2 Å3/Da / Density % sol: 44 % |
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Crystal grow | *PLUS pH: 5.3 / Method: seeding |
Components of the solutions | *PLUS Conc.: 43 %(v/v) / Common name: aqueous ethanol |
-Data collection
Reflection | *PLUS Highest resolution: 1.6 Å / Num. obs: 12742 / % possible obs: 72 % / Rmerge(I) obs: 0.033 |
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Reflection shell | *PLUS Highest resolution: 1.6 Å / Lowest resolution: 1.67 Å / % possible obs: 54 % |
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Processing
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Refinement | Rfactor obs: 0.166 / Highest resolution: 2 Å Details: THE CLOSE CONTACT BETWEEN THE ASP 83 OD2 AND ARG 85 NH2 IS DUE TO WEAK DENSITY AND DISORDER ASSOCIATED WITH THE ARG 85 RESIDUE. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement step | Cycle: LAST / Highest resolution: 2 Å
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Refine LS restraints |
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Refinement | *PLUS Rfactor obs: 0.166 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS Type: o_angle_deg / Dev ideal: 1.7 |