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Yorodumi- PDB-1sqh: X-RAY STRUCTURE OF DROSOPHILA MALONOGASTER PROTEIN Q9VR51 NORTHEA... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1sqh | ||||||
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Title | X-RAY STRUCTURE OF DROSOPHILA MALONOGASTER PROTEIN Q9VR51 NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET FR87. | ||||||
Components | hypothetical protein CG14615-PA | ||||||
Keywords | STRUCTURAL GENOMICS / UNKNOWN FUNCTION / PSI / Protein Structure Initiative / Northeast Structural Genomics Consortium / NESG | ||||||
Function / homology | Function and homology information acyltransferase activity, transferring groups other than amino-acyl groups / Transferases; Acyltransferases; Transferring groups other than aminoacyl groups Similarity search - Function | ||||||
Biological species | Drosophila melanogaster (fruit fly) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / SAD / Resolution: 2 Å | ||||||
Authors | Kuzin, A.P. / Chen, Y. / Forouhar, F. / Acton, T.B. / Xiao, R. / Cooper, B. / Ho, C.K. / Montelione, G.T. / Tong, L. / Hunt, J.F. / Northeast Structural Genomics Consortium (NESG) | ||||||
Citation | Journal: To be Published Title: X-RAY STRUCTURE OF DROSOPHILA MALONOGASTER PROTEIN Q9VR51 NORTHEAST STRUCTURAL GENOMICS CONSORTIUM TARGET FR87 Authors: Kuzin, A.P. / Chen, Y. / Forouhar, F. / Acton, T.B. / Xiao, R. / Cooper, B. / Ho, C.K. / Montelione, G.T. / Tong, L. / Hunt, J.F. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1sqh.cif.gz | 71.6 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1sqh.ent.gz | 57.4 KB | Display | PDB format |
PDBx/mmJSON format | 1sqh.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1sqh_validation.pdf.gz | 424.3 KB | Display | wwPDB validaton report |
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Full document | 1sqh_full_validation.pdf.gz | 433 KB | Display | |
Data in XML | 1sqh_validation.xml.gz | 15.3 KB | Display | |
Data in CIF | 1sqh_validation.cif.gz | 21.3 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/sq/1sqh ftp://data.pdbj.org/pub/pdb/validation_reports/sq/1sqh | HTTPS FTP |
-Related structure data
Similar structure data | |
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Other databases |
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein | Mass: 36137.445 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) Drosophila melanogaster (fruit fly) / Production host: Escherichia coli (E. coli) / References: UniProt: Q9VR51 |
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#2: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.564 Å3/Da / Density % sol: 50.18 % |
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Crystal grow | Temperature: 277 K / pH: 7 Details: 100 mM ammonium sulfate, 100 mM Tris, Spermidine , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K, pH 7.00 |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: NSLS / Beamline: X4A / Wavelength: 0.97933 |
Detector | Type: ADSC QUANTUM 4 / Detector: CCD / Date: Jan 31, 2004 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.97933 Å / Relative weight: 1 |
Reflection | Resolution: 2→30 Å / Num. obs: 43215 / % possible obs: 88.8 % / Observed criterion σ(I): -3 / Redundancy: 3.25 % / Biso Wilson estimate: 26.3 Å2 / Rmerge(I) obs: 0.109 / Rsym value: 0.109 |
Reflection shell | Resolution: 2→2.07 Å / % possible all: 49.7 |
-Processing
Software |
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Refinement | Method to determine structure: SAD / Resolution: 2→19.84 Å / Rfactor Rfree error: 0.006 / Data cutoff high absF: 346738.81 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 2 / Stereochemistry target values: ENGH & HUBER Details: 8542 REFLECTIONS ARE UNOBSERVED (NO ENTRY OR |F|=0)
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 32.42 Å2 / ksol: 0.3 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 31.3 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2→19.84 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2→2.13 Å / Rfactor Rfree error: 0.033 / Total num. of bins used: 6
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Xplor file |
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