Mass: 3397.247 Da / Num. of mol.: 1 / Source method: obtained synthetically
#2: DNA chain
SIGMA-KRNAPOLYMERASECONSENSUSSEQUENCE
Mass: 3308.185 Da / Num. of mol.: 1 / Source method: obtained synthetically
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Experimental details
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Experiment
Experiment
Method: SOLUTION NMR
NMR experiment
Conditions-ID
Experiment-ID
Solution-ID
Type
1
1
1
NOESY
1
2
1
COSY
NMR details
Text: STRUCTURE DETERMINED WITH HOMONUCLEAR NMR METHODS.
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Sample preparation
Details
Contents: H2O AND D2O
Sample conditions
Ionic strength: 0.5 M / pH: 7 / Pressure: 1 ATMOSPHERE / Temperature: 298 K
Crystal grow
*PLUS
Method: other / Details: NMR
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NMR measurement
NMR spectrometer
Type: Bruker AMX600 / Manufacturer: Bruker / Model: AMX600 / Field strength: 600 MHz
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Processing
Software
Name: AMBER / Classification: refinement
NMR software
Name
Developer
Classification
MARDIGRAS
PEARLMAN
refinement
MARDIGRAS
structuresolution
Amber
structuresolution
DNAminiCarlo
structuresolution
Refinement
Method: MOLECULAR DYNAMIC (RMD), MONTE CARLO (RMC) CALCULATIONS Software ordinal: 1 Details: REFINEMENT DETAILS CAN BE FOUND IN THE BIOCHEMISTRY CITATION ABOVE. MODEL 1 : REFINED BY RMD FROM A-DNA MODEL 2 : REFINED BY RMD FROM B-DNA MODEL 3 : REFINED BY RMC FROM A-DNA MODEL 4 : REFINED BY RMC FROM B-DNA
NMR ensemble
Conformer selection criteria: CONVERGENCE, R FACTORS, ENERGIES Conformers calculated total number: 20 / Conformers submitted total number: 4
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