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Yorodumi- PDB-1rjc: Crystal structure of the camelid single domain antibody cAb-Lys2 ... -
+Open data
-Basic information
Entry | Database: PDB / ID: 1rjc | ||||||
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Title | Crystal structure of the camelid single domain antibody cAb-Lys2 in complex with hen egg white lysozyme | ||||||
Components |
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Keywords | IMMUNE SYSTEM/HYDROLASE / beta sandwich / immunoglobulin fold / protein-protein hetero complex / alpha-beta orthogonal bundle / IMMUNE SYSTEM-HYDROLASE COMPLEX | ||||||
Function / homology | Function and homology information Antimicrobial peptides / Neutrophil degranulation / beta-N-acetylglucosaminidase activity / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / killing of cells of another organism / defense response to Gram-negative bacterium / defense response to Gram-positive bacterium / defense response to bacterium ...Antimicrobial peptides / Neutrophil degranulation / beta-N-acetylglucosaminidase activity / cell wall macromolecule catabolic process / lysozyme / lysozyme activity / killing of cells of another organism / defense response to Gram-negative bacterium / defense response to Gram-positive bacterium / defense response to bacterium / endoplasmic reticulum / extracellular space / identical protein binding / cytoplasm Similarity search - Function | ||||||
Biological species | Camelus dromedarius (Arabian camel) Gallus gallus (chicken) | ||||||
Method | X-RAY DIFFRACTION / SYNCHROTRON / MOLECULAR REPLACEMENT / Resolution: 1.4 Å | ||||||
Authors | De Genst, E. / Silence, K. / Ghahroudi, M.A. / Decanniere, K. / Loris, R. / Kinne, J. / Wyns, L. / Muyldermans, S. | ||||||
Citation | Journal: J.Biol.Chem. / Year: 2005 Title: Strong in vivo maturation compensates for structurally restricted H3 loops in antibody repertoires. Authors: De Genst, E. / Silence, K. / Ghahroudi, M.A. / Decanniere, K. / Loris, R. / Kinne, J. / Wyns, L. / Muyldermans, S. | ||||||
History |
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Remark 999 | The sequence of CAMELID heavy chain ANTIBODY (chain A) is not available in any reference sequence database. |
-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1rjc.cif.gz | 60.4 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1rjc.ent.gz | 47.4 KB | Display | PDB format |
PDBx/mmJSON format | 1rjc.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rj/1rjc ftp://data.pdbj.org/pub/pdb/validation_reports/rj/1rjc | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Antibody | Mass: 14902.298 Da / Num. of mol.: 1 / Fragment: VHH DOMAIN, VARIABLE DOMAIN Source method: isolated from a genetically manipulated source Source: (gene. exp.) Camelus dromedarius (Arabian camel) / Tissue: Blood Lymphocytes / Plasmid: pHEN06 / Production host: Escherichia coli (E. coli) / Strain (production host): WK6Su |
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#2: Protein | Mass: 14331.160 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Source: (natural) Gallus gallus (chicken) / Tissue: egg white / References: UniProt: P00698, lysozyme |
#3: Chemical | ChemComp-PO4 / |
#4: Chemical | ChemComp-GOL / |
#5: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.35 Å3/Da / Density % sol: 47.25 % |
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Crystal grow | Temperature: 298 K / Method: vapor diffusion, hanging drop / pH: 7.4 Details: PEG 8000, glycerol, ammonium sulphate, potassium phosphate, sodium chloride, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
-Data collection
Diffraction | Mean temperature: 100 K |
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Diffraction source | Source: SYNCHROTRON / Site: EMBL/DESY, Hamburg / Beamline: BW7B / Wavelength: 0.8453 Å |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: Oct 28, 2002 |
Radiation | Monochromator: SAGITALLY FOCUSED Si(111) / Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 0.8453 Å / Relative weight: 1 |
Reflection | Resolution: 1.4→49.4 Å / Num. all: 49374 / Num. obs: 48909 / % possible obs: 99.06 % / Observed criterion σ(F): 0 / Observed criterion σ(I): 0 / Redundancy: 4.5 % / Biso Wilson estimate: 13.2 Å2 / Rmerge(I) obs: 0.065 / Rsym value: 0.065 / Net I/σ(I): 21.4 |
Reflection shell | Resolution: 1.4→1.45 Å / Rmerge(I) obs: 0.371 / Mean I/σ(I) obs: 3.7 / Num. unique all: 4610 / Rsym value: 0.371 / % possible all: 96.8 |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT / Resolution: 1.4→26.88 Å / Rfactor Rfree error: 0.005 / Data cutoff high absF: 1381007.94 / Data cutoff high rms absF: 1381007.94 / Data cutoff low absF: 0 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 0 / Stereochemistry target values: Engh & Huber / Details: BULK SOLVENT MODEL USED
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Solvent computation | Solvent model: FLAT MODEL / Bsol: 43.6135 Å2 / ksol: 0.379361 e/Å3 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | Biso mean: 16.5 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 1.4→26.88 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.4→1.49 Å / Rfactor Rfree error: 0.015 / Total num. of bins used: 6
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Xplor file |
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