+Open data
-Basic information
Entry | Database: PDB / ID: 1rh4 | ||||||
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Title | RH4 DESIGNED RIGHT-HANDED COILED COIL TETRAMER | ||||||
Components | RIGHT-HANDED COILED COIL TETRAMER | ||||||
Keywords | COILED COIL / DE NOVO DESIGN | ||||||
Function / homology | ISOPROPYL ALCOHOL Function and homology information | ||||||
Biological species | synthetic construct (others) | ||||||
Method | X-RAY DIFFRACTION / MOLECULAR REPLACEMENT / Resolution: 1.9 Å | ||||||
Authors | Harbury, P.B. / Plecs, J.J. / Tidor, B. / Alber, T. / Kim, P.S. | ||||||
Citation | Journal: Science / Year: 1998 Title: High-resolution protein design with backbone freedom. Authors: Harbury, P.B. / Plecs, J.J. / Tidor, B. / Alber, T. / Kim, P.S. #1: Journal: Proc.Natl.Acad.Sci.USA / Year: 1995 Title: Repacking Protein Cores with Backbone Freedom: Structure Prediction for Coiled Coils Authors: Harbury, P.B. / Tidor, B. / Kim, P.S. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 1rh4.cif.gz | 17.7 KB | Display | PDBx/mmCIF format |
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PDB format | pdb1rh4.ent.gz | 11.6 KB | Display | PDB format |
PDBx/mmJSON format | 1rh4.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Summary document | 1rh4_validation.pdf.gz | 378.1 KB | Display | wwPDB validaton report |
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Full document | 1rh4_full_validation.pdf.gz | 378.1 KB | Display | |
Data in XML | 1rh4_validation.xml.gz | 2.2 KB | Display | |
Data in CIF | 1rh4_validation.cif.gz | 2.8 KB | Display | |
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/rh/1rh4 ftp://data.pdbj.org/pub/pdb/validation_reports/rh/1rh4 | HTTPS FTP |
-Related structure data
Similar structure data |
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-Links
-Assembly
Deposited unit |
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1 |
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Unit cell |
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-Components
#1: Protein/peptide | Mass: 3637.529 Da / Num. of mol.: 1 Source method: isolated from a genetically manipulated source Source: (gene. exp.) synthetic construct (others) | ||
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#2: Chemical | #3: Water | ChemComp-HOH / | |
-Experimental details
-Experiment
Experiment | Method: X-RAY DIFFRACTION / Number of used crystals: 1 |
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-Sample preparation
Crystal | Density Matthews: 2.5 Å3/Da / Density % sol: 52 % | ||||||||||||||||||||||||||||||
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Crystal grow | pH: 8.1 Details: 100MM TRIS PH 8.1 0.3M NABR 20% 2-PROPANOL 20% POLYETHLENE GLYCOL 1450 | ||||||||||||||||||||||||||||||
Crystal grow | *PLUS Method: vapor diffusion | ||||||||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 300 K |
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Diffraction source | Source: ROTATING ANODE / Type: RIGAKU / Wavelength: 1.5418 |
Detector | Type: RIGAKU RAXIS II / Detector: IMAGE PLATE / Date: Jun 1, 1995 |
Radiation | Monochromator: NI FILTER / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1.5418 Å / Relative weight: 1 |
Reflection | Resolution: 1.9→6 Å / Num. obs: 3175 / % possible obs: 86 % / Observed criterion σ(I): 2 / Biso Wilson estimate: 31 Å2 / Rmerge(I) obs: 0.034 |
Reflection shell | Resolution: 1.9→1.98 Å / % possible all: 58.9 |
Reflection shell | *PLUS % possible obs: 58.9 % |
-Processing
Software |
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Refinement | Method to determine structure: MOLECULAR REPLACEMENT Starting model: PREDICTED STRUCTURE Resolution: 1.9→6 Å / Data cutoff high absF: 100000 / Data cutoff low absF: 0.1 / Cross valid method: THROUGHOUT / σ(F): 2
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Refinement step | Cycle: LAST / Resolution: 1.9→6 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 1.9→1.98 Å / Total num. of bins used: 8
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Xplor file |
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Software | *PLUS Name: X-PLOR / Version: 3.1 / Classification: refinement | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor obs: 0.273 |