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Yorodumi- PDB-2ljc: Structure of the influenza AM2-BM2 chimeric channel bound to rima... -
+Open data
-Basic information
Entry | Database: PDB / ID: 2ljc | ||||||
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Title | Structure of the influenza AM2-BM2 chimeric channel bound to rimantadine | ||||||
Components | M2 protein, BM2 protein chimera | ||||||
Keywords | Transport protein/Inhibitor / M2 channel / rimantadine binding / drug complex / TRANSPORT PROTEIN / Transport protein-Inhibitor complex | ||||||
Function / homology | Function and homology information suppression by virus of host autophagy / proton transmembrane transporter activity / proton transmembrane transport / : / protein complex oligomerization / monoatomic ion channel activity / host cell plasma membrane / virion membrane / membrane / plasma membrane Similarity search - Function | ||||||
Biological species | Influenza A virus Influenza B virus | ||||||
Method | SOLUTION NMR / simulated annealing, molecular dynamics | ||||||
Model details | lowest energy, model 1 | ||||||
Authors | Pielak, R.M. / Oxenoid, K. / Chou, J.J. | ||||||
Citation | Journal: Structure / Year: 2011 Title: Structural investigation of rimantadine inhibition of the AM2-BM2 chimera channel of influenza viruses. Authors: Pielak, R.M. / Oxenoid, K. / Chou, J.J. | ||||||
History |
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-Structure visualization
Structure viewer | Molecule: MolmilJmol/JSmol |
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-Downloads & links
-Download
PDBx/mmCIF format | 2ljc.cif.gz | 566 KB | Display | PDBx/mmCIF format |
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PDB format | pdb2ljc.ent.gz | 479.7 KB | Display | PDB format |
PDBx/mmJSON format | 2ljc.json.gz | Tree view | PDBx/mmJSON format | |
Others | Other downloads |
-Validation report
Arichive directory | https://data.pdbj.org/pub/pdb/validation_reports/lj/2ljc ftp://data.pdbj.org/pub/pdb/validation_reports/lj/2ljc | HTTPS FTP |
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-Related structure data
-Links
-Assembly
Deposited unit |
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1 |
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NMR ensembles |
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-Components
#1: Protein/peptide | Mass: 3805.371 Da / Num. of mol.: 4 / Mutation: C19S Source method: isolated from a genetically manipulated source Source: (gene. exp.) Influenza A virus, Influenza B virus Strain: H3N2, B/Taiwan/70061/2006Influenza A virus subtype H3N2 Gene: BM2 / Production host: Escherichia coli (E. coli) / Strain (production host): BL21(DE3) / References: UniProt: Q9YP62, UniProt: B4UQM4 #2: Chemical | ChemComp-RIM / | |
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-Experimental details
-Experiment
Experiment | Method: SOLUTION NMR | ||||||||||||||||||||||||||||||||||||||||||||||||
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NMR experiment |
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-Sample preparation
Details |
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Sample |
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Sample conditions | Ionic strength: 100 / pH: 7.5 / Pressure: ambient / Temperature: 303 K |
-NMR measurement
NMR spectrometer | Type: Bruker DMX / Manufacturer: Bruker / Model: DMX / Field strength: 600 MHz |
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-Processing
NMR software |
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Refinement | Method: simulated annealing, molecular dynamics / Software ordinal: 1 | ||||||||||||||||||
NMR representative | Selection criteria: lowest energy | ||||||||||||||||||
NMR ensemble | Conformer selection criteria: structures with the lowest energy Conformers calculated total number: 75 / Conformers submitted total number: 15 |