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Open data
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Basic information
Entry | Database: PDB / ID: 1qti | ||||||
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Title | Acetylcholinesterase (E.C.3.1.1.7) | ||||||
![]() | ACETYLCHOLINESTERASE | ||||||
![]() | HYDROLASE / ALZHEIMER'S DISEASE / DRUG / SERINE HYDROLASE / ALPHA/BETA HYDROLASE / NEUROTRANSMITTER CLEAVEAGE / CATALYTIC TRIAD | ||||||
Function / homology | ![]() acetylcholine catabolic process in synaptic cleft / acetylcholinesterase / acetylcholinesterase activity / choline metabolic process / side of membrane / synaptic cleft / synapse / extracellular space / plasma membrane Similarity search - Function | ||||||
Biological species | ![]() ![]() | ||||||
Method | ![]() ![]() | ||||||
![]() | Bartolucci, C. / Perola, E. / Pilger, C. / Fels, G. / Lamba, D. | ||||||
![]() | ![]() Title: Three-dimensional structure of a complex of galanthamine (Nivalin) with acetylcholinesterase from Torpedo californica: implications for the design of new anti-Alzheimer drugs Authors: Bartolucci, C. / Perola, E. / Pilger, C. / Fels, G. / Lamba, D. #1: ![]() Title: Atomic Structure of Acetylcholinesterase from Torpedo californica: A Prototypic Acetylcholine-Binding Protein Authors: Sussman, J.L. / Harel, M. / Frolow, F. / Oefner, C. / Goldman, A. / Toker, L. / Silman, I. #2: ![]() Title: Potent Acetylcholinesterase Inhibitors-Design, Synthesis, and Structure- Activity Relationships of Bis-Interacting Ligands in the Galanthamine Series Authors: Mary, A. / Renko, D.Z. / Guillou, C. / Thal, C. #3: ![]() Title: New Kilogram-Synthesis of the Anti-Alzheimer Drug (-)-Galanthamine Authors: Czollner, L. / Frantsits, W. / Kuenburg, B. / Hedenig, U. / Frohlich, J. / Jordis, U. #4: ![]() Title: Prediction of the Binding Site of (-)-Galanthamine in Acetylcholinesterase by Docking Studies Authors: Pilger, C. / Bartolucci, C. / Lamba, D. / Tropsha, A. / Fels, G. | ||||||
History |
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Structure visualization
Structure viewer | Molecule: ![]() ![]() |
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Downloads & links
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Download
PDBx/mmCIF format | ![]() | 116.4 KB | Display | ![]() |
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PDB format | ![]() | 95.2 KB | Display | ![]() |
PDBx/mmJSON format | ![]() | Tree view | ![]() | |
Others | ![]() |
-Validation report
Summary document | ![]() | 786.7 KB | Display | ![]() |
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Full document | ![]() | 801.6 KB | Display | |
Data in XML | ![]() | 24 KB | Display | |
Data in CIF | ![]() | 33.5 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | |
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Similar structure data |
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Links
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Assembly
Deposited unit | ![]()
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1 | ![]()
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Unit cell |
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Components
#1: Protein | Mass: 60736.516 Da / Num. of mol.: 1 / Source method: isolated from a natural source / Details: COMPLEXED WITH GALANTHAMINE Source: (natural) ![]() ![]() References: UniProt: P04058, acetylcholinesterase |
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#2: Chemical | ChemComp-GNT / (-)- |
#3: Water | ChemComp-HOH / |
-Experimental details
-Experiment
Experiment | Method: ![]() |
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Sample preparation
Crystal | Density Matthews: 3.98 Å3/Da / Density % sol: 69.1 % | |||||||||||||||||||||||||
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Crystal grow | Temperature: 277 K / Method: vapor diffusion, hanging drop Details: 40% PEG 200, 100MM MES (pH 6.0), VAPOR DIFFUSION, HANGING DROP, temperature 277K | |||||||||||||||||||||||||
Crystal grow | *PLUS pH: 6 / Details: Sussman, J.L., (1988) J. Mol. Biol., 203, 821. | |||||||||||||||||||||||||
Components of the solutions | *PLUS
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-Data collection
Diffraction | Mean temperature: 120 K |
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Diffraction source | Source: ![]() ![]() ![]() |
Detector | Type: MARRESEARCH / Detector: IMAGE PLATE / Date: May 13, 1998 |
Radiation | Protocol: SINGLE WAVELENGTH / Monochromatic (M) / Laue (L): M / Scattering type: x-ray |
Radiation wavelength | Wavelength: 1 Å / Relative weight: 1 |
Reflection | Resolution: 2.3→20 Å / Num. all: 42574 / Num. obs: 38494 / % possible obs: 96.1 % / Observed criterion σ(I): -3 / Redundancy: 4.4 % / Biso Wilson estimate: 32.6 Å2 / Rmerge(I) obs: 0.071 / Net I/σ(I): 7.7 |
Reflection shell | Resolution: 2.3→2.4 Å / Redundancy: 2.8 % / Rmerge(I) obs: 0.21 / % possible all: 85.9 |
Reflection | *PLUS Num. obs: 42574 / Observed criterion σ(I): 0 / Num. measured all: 188745 |
Reflection shell | *PLUS % possible obs: 85.9 % / Num. unique obs: 5493 / Rmerge(I) obs: 0.21 / Mean I/σ(I) obs: 3.4 |
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Processing
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Refinement | Resolution: 2.5→20 Å / Rfactor Rfree error: 0.006 / Data cutoff high absF: 1000000 / Data cutoff low absF: 0.01 / Isotropic thermal model: RESTRAINED / Cross valid method: THROUGHOUT / σ(F): 2 / Stereochemistry target values: ENGH & HUBER Details: IN THE LAST FEW REFINEMENT CYCLES VERY WEAK REFLECTIONS WITH A RATIO FCALC/FOBS GREATER THAN 2.5, CLEARLY AFFECTED BY SYSTEMATIC MEASUREMENTS ERRORS, WERE EXCLUDED FROM THE CALCULATIONS.
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Displacement parameters | Biso mean: 33.2 Å2
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Refine analyze |
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Refinement step | Cycle: LAST / Resolution: 2.5→20 Å
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Refine LS restraints |
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LS refinement shell | Resolution: 2.5→2.66 Å / Rfactor Rfree error: 0.019 / Total num. of bins used: 6
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Xplor file | Serial no: 1 / Param file: PROTEIN_REP.PARAM / Topol file: TOPHCSDX.PRO | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Software | *PLUS Name: ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refinement | *PLUS Lowest resolution: 8 Å / σ(F): 2 / % reflection Rfree: 5 % | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Solvent computation | *PLUS | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Displacement parameters | *PLUS Biso mean: 33.2 Å2 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Refine LS restraints | *PLUS
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LS refinement shell | *PLUS Rfactor Rfree: 0.275 / % reflection Rfree: 5.1 % / Rfactor Rwork: 0.269 |